GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE14862 Query DataSets for GSE14862
Status Public on Feb 19, 2009
Title Pathogenic Natural Antibodies Recognizing Annexin IV Are Required to Develop Intestinal Ischemia-Reperfusion Injury
Platform organism Mus musculus
Sample organisms Mus musculus; unidentified
Experiment type Protein profiling by protein array
Summary Intestinal ischemia-reperfusion (IR) injury is initiated when natural IgM antibodies recognize neo-epitopes that are revealed on ischemic cells. The target molecules and mechanisms whereby these neo-epitopes become accessible to recognition are not well understood. Proposing that isolated intestinal epithelial cells (IEC) may carry IR-related neo-epitopes, we used in vitro IEC binding assays to screen hybridomas created from B cells of unmanipulated wild type C57BL/6 mice. We identified a novel IgM monoclonal antibody (mAb B4) that reacted with the surface of IEC by flow cytometric analysis and was alone capable of causing complement activation, neutrophil recruitment and intestinal injury in otherwise IR-resistant Rag1-/- mice. Monoclonal Ab B4 was found to specifically recognize mouse annexin IV. Pre-injection of recombinant annexin IV blocked IR injury in wild type C57BL/6 mice, demonstrating the requirement for recognition of this protein in order to develop IR injury in the context of a complex natural antibody repertoire. Humans were also found to exhibit IgM natural antibodies that recognize annexin IV. These data in toto identify annexin IV as a key ischemia-related target antigen that is recognized by natural Abs in a pathologic process required in vivo to develop intestinal IR injury.

Keywords: Natural antibodies, Annexin, Ischemia Reperfusion, Inflammation, Complement
Overall design In the study presented here, a total of 4 custom spotted antigen slides were hybridized with known antibodies.
Contributor(s) Kulik L, Fleming SD, Moratz C, Reuter JW, Novikov A, Chen K, Andrews KA, Markaryan A, Quigg RJ, Silverman GJ, Tsokos GC, Holers VM
Citation(s) 19380783
Submission date Feb 17, 2009
Last update date Mar 20, 2012
Contact name Gregg Silverman
Phone 8585345439
Fax 8588225230
Organization name UC San Diego School of Medicine
Department Medicine
Lab B Cell Immunobiology
Street address 9500 Gilman Dr
City La Jolla
State/province CA
ZIP/Postal code 92093-0663
Country USA
Platforms (1)
GPL8165 Mus musculus spotted antigen array
Samples (4)
GSM371851 B4 Monoclonal IgM Antibody
GSM371852 NC17-D8 B4 Isotype Monoclonal IgM Antibody
GSM371853 Polyclonal IgM Antibody
BioProject PRJNA112027

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE14862_RAW.tar 450.0 Kb (http)(custom) TAR (of GPR)
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap