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Status |
Public on Jun 29, 2020 |
Title |
Heme oxygenase-1 orchestrates the immunosuppressive program of tumor-associated macrophages [ATAC-Seq] |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
Tumor-Associated Macrophages (TAMs) contribute to the maintenance of a strong immunosuppressive environment, supporting tumor progression and resistance to treatment. To date, the mechanisms that drive acquisition of these immunosuppressive features are still poorly defined. Heme oxygenase-1 (HO-1) is the rate-limiting enzyme that catabolizes free heme. It displays important cytoprotective, anti-inflammatory and antioxidant properties. A growing body of evidence suggests that HO-1 may also promote tumor development. Herein, we show that HO-1 is highly expressed in monocytic cells in the tumor microenvironment (TME) once they differentiate into TAMs. Deletion of HO-1 in the myeloid compartment enhances the beneficial effects of a therapeutic antitumor vaccine by restoring CD8 T-cell proliferation and cytotoxicity. We further show that induction of HO-1 plays a major role on monocyte education by tumor cells by modulating their transcriptional and epigenetic programs. These results identify HO-1 as a valuable therapeutic target to reprogram the TME and synergize with current cancer therapies to facilitate antitumoral response.
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Overall design |
ATAC-seq in mouse macrophages from HO1-KO and wild type samples
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Contributor(s) |
Alaluf E, Vokaer B, Detavernier A, Azouz A, Splittgerber M, Carrette A, Boon L, Libert F, Soares M, Le Moine A, Goriely S |
Citation(s) |
32369450 |
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Submission date |
Apr 13, 2020 |
Last update date |
Jun 29, 2020 |
Contact name |
Stanislas Goriely |
E-mail(s) |
stgoriel@ulb.ac.be
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Phone |
+3226509586
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Organization name |
Université Libre de Bruxelles
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Lab |
IMI
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Street address |
Rue Adrienne Bolland 8
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City |
Gosselies |
ZIP/Postal code |
B-6041 |
Country |
Belgium |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (4)
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This SubSeries is part of SuperSeries: |
GSE148585 |
Heme oxygenase-1 orchestrates the immunosuppressive program of tumor-associated macrophages |
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Relations |
BioProject |
PRJNA625081 |
SRA |
SRP256291 |