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Series GSE148582 Query DataSets for GSE148582
Status Public on Jun 29, 2020
Title Heme oxygenase-1 orchestrates the immunosuppressive program of tumor-associated macrophages [ATAC-Seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Tumor-Associated Macrophages (TAMs) contribute to the maintenance of a strong immunosuppressive environment, supporting tumor progression and resistance to treatment. To date, the mechanisms that drive acquisition of these immunosuppressive features are still poorly defined. Heme oxygenase-1 (HO-1) is the rate-limiting enzyme that catabolizes free heme. It displays important cytoprotective, anti-inflammatory and antioxidant properties. A growing body of evidence suggests that HO-1 may also promote tumor development. Herein, we show that HO-1 is highly expressed in monocytic cells in the tumor microenvironment (TME) once they differentiate into TAMs. Deletion of HO-1 in the myeloid compartment enhances the beneficial effects of a therapeutic antitumor vaccine by restoring CD8 T-cell proliferation and cytotoxicity. We further show that induction of HO-1 plays a major role on monocyte education by tumor cells by modulating their transcriptional and epigenetic programs. These results identify HO-1 as a valuable therapeutic target to reprogram the TME and synergize with current cancer therapies to facilitate antitumoral response.
 
Overall design ATAC-seq in mouse macrophages from HO1-KO and wild type samples
 
Contributor(s) Alaluf E, Vokaer B, Detavernier A, Azouz A, Splittgerber M, Carrette A, Boon L, Libert F, Soares M, Le Moine A, Goriely S
Citation(s) 32369450
Submission date Apr 13, 2020
Last update date Jun 29, 2020
Contact name Stanislas Goriely
E-mail(s) stgoriel@ulb.ac.be
Phone +3226509586
Organization name Université Libre de Bruxelles
Lab IMI
Street address Rue Adrienne Bolland 8
City Gosselies
ZIP/Postal code B-6041
Country Belgium
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (4)
GSM4474532 ATAC_Macrophages_KO1-HO1
GSM4474533 ATAC_Macrophages_KO2-HO1
GSM4474534 ATAC_Macrophages_WT1
This SubSeries is part of SuperSeries:
GSE148585 Heme oxygenase-1 orchestrates the immunosuppressive program of tumor-associated macrophages
Relations
BioProject PRJNA625081
SRA SRP256291

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE148582_RAW.tar 578.4 Mb (http)(custom) TAR (of BIGWIG, NARROWPEAK)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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