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Series GSE14833 Query DataSets for GSE14833
Status Public on Feb 13, 2010
Title Expression data from different stages of hematopoietic cells development
Organism Mus musculus
Experiment type Expression profiling by array
Summary 18 different population of cells in different developmental stages in hematopoietic hierarchy have been purifyed by FACS analyses from wild type C57Bl6 mice and subjected to Micrroarray Affymetrix mouse 430.2 platform
We used microarrays to compare global expression pattern in different hematopoietic cell populations to reconstruct the whole hierarchy of hematopoietic tree based on gene profiling

Keywords: Hematopoietic Development
 
Overall design For analysis of progenitor populations, RNA was extracted from 2000 purified adult BM cells using RNAeasy microkit. RNA was labeled and amplified by dual amplification and hybridized to Affymetrix micro array u480A, according to AffymetrixTM GeneChip Expression Analysis Technical Manual. Probe level expression values were calculated using RMA
 
Contributor(s) Zandi S, Sigvardsson M, Mansson R, Bryder D
Citation(s) 21969581
Submission date Feb 13, 2009
Last update date Feb 11, 2019
Contact name Sasan Zandi
E-mail sazandi@gmail.com
Organization name UHN
Street address 101 college St
City Toronto
State/province Ontario
ZIP/Postal code M5G1L7
Country Canada
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (47)
GSM371211 CD4 Adult bone marrow, biological replicate 1
GSM371212 CD4 Adult bone marrow, biological replicate 2
GSM371213 CFUE Adult bone marrow, biological replicate 1
Relations
BioProject PRJNA112073

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE14833_RAW.tar 225.1 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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