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| Status |
Public on May 13, 2020 |
| Title |
HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription [ChIP-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Functional crosstalk between histone modifications and chromatin remodeling has emerged as a key regulatory mode of transcriptional control during cell fate decisions, but the underlying mechanisms are not fully understood. Here we demonstrate that HRP2-DPF3a-BAF complex coordinates histone H3 lysine 36 methylation (H3K36me) and ATP-dependent chromatin remodeling to regulate chromatin dynamic and gene transcription during myogenic differentiation. Mechanistically, through its HIV integrase binding domain (IBD), HRP2 associates with BRG1/BRM associated factor (BAF) chromatin remodeling complex by direct interaction with BAF45c (DPF3a) subunit. Through its Pro-Trp-Trp-Pro (PWWP) domain, HRP2 preferentially binds to H3K36me2. Consistent with the biochemical studies, genome-wide analyses show that HRP2 colocalizes with DPF3a at gene promoters enriched for H3K36me2.
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| Overall design |
Examination of binding of Hrp2 in mouse myoblasts and myotubes by ChIP-seq. Examination of binding of Dpf3a and H3K36me2 in mouse myotubes by ChIP-seq.
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| Contributor(s) |
Zhu X, Lan B, Yi X |
| Citation(s) |
32459350 |
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| Submission date |
Apr 08, 2020 |
| Last update date |
Jul 20, 2020 |
| Contact name |
Bingxue Lan |
| Organization name |
Tianjin Medical University
|
| Street address |
Qixiangtai road
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| City |
Tianjin |
| ZIP/Postal code |
300070 |
| Country |
China |
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| Platforms (1) |
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| Samples (21)
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| This SubSeries is part of SuperSeries: |
| GSE141407 |
HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription |
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| Relations |
| BioProject |
PRJNA623795 |
| SRA |
SRP255719 |
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