|
| Status |
Public on Jun 08, 2020 |
| Title |
Total RNA sequencing of postnatal male germ cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report the whole-transcriptome profile with total RNA sequencing of postnatal male germ cells.
Histone modification levels are dynamically controlled during mammalian spermatogenesis. We found that H3K9 demethylases, Jmjd1a and Jmjd1b catalyze H3K9 demethylation in prospermatogonia. Combined loss of Jmjd1 enzymes disturbed prospermatogonia to spermatogonia transition in mice. To examine a role of Jmjd1 in prospermatogonia to spermatogonia transition, we performed RNA-seq and ChIP-seq analyses using postnatal germ cells at P3 and P7.
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| |
|
| Overall design |
mRNA profiles of postnatal male germ cells isolated from testes of a series of Vasa-Cre; Jmjd1; Jmjd1b conditional knockout mice
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| |
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| Contributor(s) |
Kuroki S, Maeda R, Tachibana M |
| Citation(s) |
32679061 |
| |
| Submission date |
Apr 03, 2020 |
| Last update date |
May 07, 2025 |
| Contact name |
Ryo Maeda |
| E-mail(s) |
rmaeda.fbs@osaka-u.ac.jp
|
| Organization name |
The University of Osaka
|
| Street address |
Yamadaoka 1-3
|
| City |
Suita-shi, Osaka |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
| |
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| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
|
| Samples (14)
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| This SubSeries is part of SuperSeries: |
| GSE148055 |
RNA-seq and H3K9me2 ChIP-seq of postnatal male germ cells |
|
| Relations |
| BioProject |
PRJNA622968 |
| SRA |
SRP255165 |
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