|
| Status |
Public on Sep 16, 2011 |
| Title |
An integrative multi-platform analysis for biomarker discovery of osteosarcoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Three osteosarcoma (OS) cell lines (MG-63, Saos-2 and U-2 OS) and 1 osteoblastic cell line (hFOB1.19) were collected for this work. MG-63 was kindly provided by Dr. Agi Grigoriadis from University College London. Saos-2, U-2 OS and hFOB1.19 were purchased from ATCC. All cells used were kept in exponential phase of growth. Total RNA was extracted using the RNeasy Total RNA Isolation kit (QIAGEN). The quality and purity of the products were controlled by Agilent 2100. The final synthesized biotinylated cDNAs were hybridized to Affymetrix GeneChip® U133A 2.0 arrays following the protocol strictly. Arrays were scanned with the Affymetrix scanner 3000. Data analysis was performed by Microarray Suite 5.0 after pre-standard procedure.
|
| |
|
| Overall design |
Link-test on datasets from both SELDI-TOF-MS and microarray high-throughput analysis platforms can accelerate the identification of tumor biomarkers. The results confirmed that CYC-1 with important biomedical function was an effective candidate biomarker for osteosarcoma early diagnosis.
|
| |
|
| Contributor(s) |
Li DG, Zhang JW, Zeng zH, Liu LJ |
| Citation(s) |
19445706 |
| |
| Submission date |
Feb 11, 2009 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Long Ji Liu |
| E-mail(s) |
jilong.liu@ymail.com
|
| Phone |
+8605922187356
|
| Fax |
+8605922187356
|
| URL |
http://www.liubros.cn
|
| Organization name |
NEAU
|
| Street address |
59# Daxue Road
|
| City |
Xiamen |
| ZIP/Postal code |
361005 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA112223 |
Less...
More...