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Series GSE147747 Query DataSets for GSE147747
Status Public on May 03, 2020
Title Molecular Atlas of the Adult Mouse Brain
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Brain maps are essential for integrating information and interpreting the structure-function relationship of circuits and behavior. We aimed to generate a systematic classification of the adult mouse brain organization based on unbiased extraction of spatially-defining features. Applying whole-brain spatial transcriptomics, we captured the gene expression signatures to define the spatial organization of molecularly discrete subregions. We found that the molecular code contained sufficiently detailed information to directly deduce the complex spatial organization of the brain. This unsupervised molecular classification revealed new area- and layer-specific subregions, for example in isocortex and hippocampus, and a new division of striatum. The whole-brain molecular atlas further supports the identification of the spatial origin of single neurons using their gene expression profile, and forms the foundation to define a minimal gene set - a brain palette – that is sufficient to spatially annotate the adult brain. In summary, we have established a new molecular atlas to formally define the identity of brain regions, and a molecular code for mapping and targeting of discrete neuroanatomical domains.
Overall design We hybridized 75 coronal sections from one brain hemisphere of 3 adult mice with Spatial Transcriptomics and defined a molecular atlas using clustering algorithms.
Contributor(s) Ortiz C, Fernandez Navarro J, Jurek A, Märtin A, Lundeberg J, Meletis K
Citation(s) 32637622
Submission date Mar 30, 2020
Last update date Jul 13, 2020
Contact name Konstantinos Meletis
Organization name Karolinska Institutet
Department Neuroscience
Lab Meletis lab
Street address Solnavägen 9
City Stockholm
ZIP/Postal code 171 65
Country Sweden
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (75)
GSM4459917 01A
GSM4459918 01B
GSM4459919 02A
BioProject PRJNA623858
SRA SRP255730

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SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE147747_RAW.tar 550.3 Mb (http)(custom) TAR (of JPG, TSV)
GSE147747_expr_normalized_table.tsv.gz 1.9 Gb (ftp)(http) TSV
GSE147747_expr_raw_counts_table.tsv.gz 155.8 Mb (ftp)(http) TSV
GSE147747_meta_table.tsv.gz 1.5 Mb (ftp)(http) TSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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