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| Status |
Public on Oct 11, 2020 |
| Title |
Single-nucleus RNA-seq identifies transcriptional heterogeneity in multinucleated skeletal myofibers |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
While the majority of cells contain a single nucleus, cell types such as trophoblasts, osteoclasts, and skeletal myofibers require multinucleation. One advantage of multinucleation can be the assignment of distinct functions to different nuclei, but comprehensive interrogation of transcriptional heterogeneity within multinucleated tissues has been challenging due to the presence of a shared cytoplasm. Here, we utilized single-nucleus RNA-sequencing (snRNA-seq) to determine the extent of transcriptional diversity within multinucleated skeletal myofibers. Nuclei from mouse skeletal muscle were profiled across the lifespan, which revealed the emergence of distinct myonuclear populations in postnatal development and their reactivation in aging muscle. Our datasets also provided a platform for discovery of novel genes associated with rare specialized regions of the muscle cell, including markers of the myotendinous junction and functionally validated factors expressed at the neuromuscular junction. These findings reveal that myonuclei within syncytial muscle fibers possess distinct transcriptional profiles that regulate muscle biology.
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| Overall design |
Single-nucleus RNA-seq of tibialis anteior muscle of BL6 wild-type mice across the lifespan: 10 days, 21 days, 5 months (TA and soleus), 24 months, 30 months
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| |
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| Contributor(s) |
Petrany MJ, Swoboda CO, Sun C, Chetal K, Chen X, Weirauch MT, Salomonis N, Millay DP |
| Citation(s) |
33311464 |
| |
| Submission date |
Mar 17, 2020 |
| Last update date |
Aug 09, 2022 |
| Contact name |
Nathan Salomonis |
| E-mail(s) |
nathan.salomonis@cchmc.org
|
| Organization name |
Cincinnati Children's Hospital
|
| Department |
Biomedical Informatics
|
| Lab |
Nathan Salomonis
|
| Street address |
3333 Burnet Avenue
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (6)
|
| GSM4418991 |
WT tibialis anterior 24 months 10X snRNA-seq |
| GSM4418992 |
C57BL/6 WT tibialis anterior 5 months 10X snRNA-seq |
| GSM4418993 |
C57BL/6 WT tibialis anterior 30 months 10X snRNA-seq |
| GSM4418994 |
C57BL/6 WT tibialis anterior postnatal day 10 10X snRNA-seq |
| GSM4418995 |
C57BL/6 WT tibialis anterior postnatal day 21 10X snRNA-seq |
| GSM4418996 |
C57BL/6 WT soleus 5 months 10X snRNA-seq |
|
| Relations |
| BioProject |
PRJNA613077 |
| SRA |
SRP253104 |
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