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Series GSE147127 Query DataSets for GSE147127
Status Public on Oct 11, 2020
Title Single-nucleus RNA-seq identifies transcriptional heterogeneity in multinucleated skeletal myofibers
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary While the majority of cells contain a single nucleus, cell types such as trophoblasts, osteoclasts, and skeletal myofibers require multinucleation. One advantage of multinucleation can be the assignment of distinct functions to different nuclei, but comprehensive interrogation of transcriptional heterogeneity within multinucleated tissues has been challenging due to the presence of a shared cytoplasm. Here, we utilized single-nucleus RNA-sequencing (snRNA-seq) to determine the extent of transcriptional diversity within multinucleated skeletal myofibers. Nuclei from mouse skeletal muscle were profiled across the lifespan, which revealed the emergence of distinct myonuclear populations in postnatal development and their reactivation in aging muscle. Our datasets also provided a platform for discovery of novel genes associated with rare specialized regions of the muscle cell, including markers of the myotendinous junction and functionally validated factors expressed at the neuromuscular junction. These findings reveal that myonuclei within syncytial muscle fibers possess distinct transcriptional profiles that regulate muscle biology.
Overall design Single-nucleus RNA-seq of tibialis anteior muscle of BL6 wild-type mice across the lifespan: 10 days, 21 days, 5 months (TA and soleus), 24 months, 30 months
Contributor(s) Petrany MJ, Swoboda CO, Sun C, Chetal K, Chen X, Weirauch MT, Salomonis N, Millay DP
Citation(s) 33311464
Submission date Mar 17, 2020
Last update date Aug 09, 2022
Contact name Nathan Salomonis
Organization name Cincinnati Children's Hospital
Department Biomedical Informatics
Lab Nathan Salomonis
Street address 3333 Burnet Avenue
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM4418991 WT tibialis anterior 24 months 10X snRNA-seq
GSM4418992 C57BL/6 WT tibialis anterior 5 months 10X snRNA-seq
GSM4418993 C57BL/6 WT tibialis anterior 30 months 10X snRNA-seq
BioProject PRJNA613077
SRA SRP253104

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Supplementary file Size Download File type/resource
GSE147127_RAW.tar 643.7 Mb (http)(custom) TAR (of H5)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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