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| Status |
Public on Mar 09, 2021 |
| Title |
Single Cell Sequencing Reveals Early PGC-like Intermediates During Mouse Primed to Naïve Transition |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Single cell analysis provides clarity unattainable with bulk approaches. Here we apply single cell RNA-seq to a newly established BMP4 induced mouse primed to naive transition (Bi-PNT) system and show that the reset is not a direct reversal of cell fate but through developmental intermediates. We first show that mEpiSCs bifurcate into c-Kit+ naïve and c-Kit- placenta-like cells, among which, the naive branch undergoes further transition through a primordial germ cell-like cells (PGCLCs) intermediate capable of spermatogenesis in vivo. Indeed, deficiency of Prdm1/Blimp1, the key regulator for PGC specification, blocks the induction of PGCLCs and naïve cells. Instead, Gata2 knockout arrests placenta-like fate, but facilitates the generation of PGCLCs. Our results not only reveal a newly cell fate dynamics between primed and naive states at single-cell resolution, but also provide a model system to explore mechanisms involved in regaining germline competence from primed pluripotency.
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| Overall design |
We analyzed the single-cell transcriptome expression profiles (scRNA-seq) during mouse primed naïve transition (PNT).
We analyzed the transcriptome expression of BV+SC+ cells generated during mouse primed to naïve transition.
We analyzed the transcriptome expression and chromatin remodelling of cKit positive and negative cells during mouse primed naïve transition (PNT).
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| Web link |
https://assets.researchsquare.com/files/rs-151340/v1_stamped.pdf
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| Contributor(s) |
He J |
| Citation(s) |
35589713 |
| |
| Submission date |
Mar 17, 2020 |
| Last update date |
May 27, 2022 |
| Contact name |
Jiangping He |
| E-mail(s) |
he_jiangping@grmh-gdl.cn
|
| Organization name |
Guangzhou Institutes of Biomedicine and Health (GIBH), CAS
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| Street address |
Kai yuan avenue 190
|
| City |
GuangZhou |
| ZIP/Postal code |
510530 |
| Country |
China |
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| Platforms (2) |
| GPL21273 |
HiSeq X Ten (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA613024 |
| SRA |
SRP253182 |
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