|
| Status |
Public on Mar 12, 2020 |
| Title |
Chromatin accessibility dynamics and single cell RNA-Seq reveal new regulators of regeneration in neural progenitors |
| Organism |
Xenopus tropicalis |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Vertebrate appendage regeneration requires precisely coordinated remodeling of the transcriptional landscape to enable the growth and differentiation of new tissue, a process executed over multiple days and across dozens of cell types. The heterogeneity of tissues and temporally-sensitive fate decisions involved has made it difficult to articulate the gene regulatory programs enabling regeneration of individual cell types. To better understand how a regenerative program is fulfilled by neural progenitor cells (NPCs) of the spinal cord, we analyzed pax6-expressing NPCs isolated from regenerating Xenopus tropicalis tails. By intersecting chromatin accessibility data with single-cell transcriptomics, we find that NPCs place an early priority on neuronal differentiation. Late in regeneration, the priority returns to proliferation. Our analyses identify Pbx3 and Meis1 as critical regulators of tail regeneration and axon organization. Overall, we use transcriptional regulatory dynamics to present a new model for cell fate decisions and their regulators in NPCs during regeneration.
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| |
|
| Overall design |
Investigation of chromatin accessiblity dynamics in neural progenitor cells over the course of tadpole tail regeneration
|
| |
|
| Contributor(s) |
Anneke K, Alex C |
| Citation(s) |
32338593 |
| |
| Submission date |
Mar 11, 2020 |
| Last update date |
Jun 11, 2020 |
| Contact name |
Anneke Dixie Kakebeen |
| E-mail(s) |
akakebee@uw.edu
|
| Organization name |
University of Washington
|
| Department |
Biochemistry
|
| Lab |
Andrea Wills
|
| Street address |
5611 9th Ave NW
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98107 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24884 |
Illumina NextSeq 500 (Xenopus tropicalis) |
|
| Samples (31)
|
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| This SubSeries is part of SuperSeries: |
| GSE146837 |
Chromatin accessibility dynamics and single cell RNA-Seq reveal new regulators of regeneration in neural progenitors |
|
| Relations |
| BioProject |
PRJNA612063 |
| SRA |
SRP252458 |
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