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Series GSE146773 Query DataSets for GSE146773
Status Public on Dec 11, 2020
Title Single-cell temporal transcriptomics of the human cell cycle
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We present the first comprehensive map of spatiotemporal heterogeneity of the human proteome by integrating proteomics at subcellular resolution, single-cell transcriptomics, and pseudotime measurements of each cell within the cell cycle. The cell cycle transcriptomic data in this series provide context for single-cell proteomic measurements of the cell cycle, and this subsequent single-cell proteogenomic analysis that can be found in this code repository: https://github.com/CellProfiling/SingleCellProteogenomics .
 
Overall design A total of 1,152 single U2OS FUCCI cells were sorted by FACS, and RNA-Seq was collected for each cell using SMART-seq2 chemistry.
Web link https://www.nature.com/articles/s41586-021-03232-9
 
Contributor(s) Mahdessian D, Cesnik AJ, Gnann C, Danielsson F, Stenström L, Arif M, Zhang C, Le T, Johansson F, Shutten R, Bäckström A, Axelsson U, Thul P, Cho NH, Carja O, Uhlén M, Mardinoglu A, Stadler C, Lindskog C, Ayoglu B, Leonetti MD, Pontén F, Sullivan DP, Lundberg E
Citation https://doi.org/10.1101/543231
Submission date Mar 11, 2020
Last update date Mar 02, 2021
Contact name Emma Lundberg
E-mail(s) emma.lundberg@scilifelab.se
Phone +4670333363
Organization name KTH Royal Institute of Technology
Lab Lundberg
Street address Science for Life Laboratory, Tomtebodavägen 23A
City Solna
ZIP/Postal code 17121
Country Sweden
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (1152)
GSM4405639 Single U2OS cell A10_355
GSM4405640 Single U2OS cell A10_356
GSM4405641 Single U2OS cell A10_357
Relations
BioProject PRJNA611937
SRA SRP252355

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE146773_Counts.csv.ercc.csv.gz 42.4 Kb (ftp)(http) CSV
GSE146773_Counts.csv.gz 26.5 Mb (ftp)(http) CSV
GSE146773_RAW.tar 1.0 Gb (http)(custom) TAR (of TXT)
GSE146773_Tpms.csv.ercc.csv.gz 72.0 Kb (ftp)(http) CSV
GSE146773_Tpms.csv.gz 37.6 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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