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| Status |
Public on Dec 11, 2020 |
| Title |
Single-cell temporal transcriptomics of the human cell cycle |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We present the first comprehensive map of spatiotemporal heterogeneity of the human proteome by integrating proteomics at subcellular resolution, single-cell transcriptomics, and pseudotime measurements of each cell within the cell cycle. The cell cycle transcriptomic data in this series provide context for single-cell proteomic measurements of the cell cycle, and this subsequent single-cell proteogenomic analysis that can be found in this code repository: https://github.com/CellProfiling/SingleCellProteogenomics .
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| Overall design |
A total of 1,152 single U2OS FUCCI cells were sorted by FACS, and RNA-Seq was collected for each cell using SMART-seq2 chemistry.
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| Contributor(s) |
Mahdessian D, Cesnik AJ, Gnann C, Danielsson F, Stenström L, Arif M, Zhang C, Le T, Johansson F, Shutten R, Bäckström A, Axelsson U, Thul P, Cho NH, Carja O, Uhlén M, Mardinoglu A, Stadler C, Lindskog C, Ayoglu B, Leonetti MD, Pontén F, Sullivan DP, Lundberg E |
| Citation(s) |
33627808 |
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https://doi.org/10.1101/543231
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| Submission date |
Mar 11, 2020 |
| Last update date |
Oct 18, 2023 |
| Contact name |
Emma Lundberg |
| E-mail(s) |
emma.lundberg@scilifelab.se
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| Phone |
+4670333363
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| Organization name |
KTH Royal Institute of Technology
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| Lab |
Lundberg
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| Street address |
Science for Life Laboratory, Tomtebodavägen 23A
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| City |
Solna |
| ZIP/Postal code |
17121 |
| Country |
Sweden |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (1152)
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| Relations |
| BioProject |
PRJNA611937 |
| SRA |
SRP252355 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE146773_Counts.csv.ercc.csv.gz |
42.4 Kb |
(ftp)(http) |
CSV |
| GSE146773_Counts.csv.gz |
26.5 Mb |
(ftp)(http) |
CSV |
| GSE146773_RAW.tar |
1.0 Gb |
(http)(custom) |
TAR (of TXT) |
| GSE146773_Tpms.csv.ercc.csv.gz |
72.0 Kb |
(ftp)(http) |
CSV |
| GSE146773_Tpms.csv.gz |
37.6 Mb |
(ftp)(http) |
CSV |
| GSE146773_fucci_coords.csv.gz |
41.5 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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