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| Status |
Public on Jan 18, 2021 |
| Title |
CTCF is required to form regulatory and physical boundaries at limited sites in the Drosophila nervous system [Hi-C] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
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| Summary |
CCCTC-binding factor (CTCF) is a conserved protein able to block communication between regulatory elements and gene promoters in flies and mammals. Here, we studied CTCF function in the Drosophila central nervous system (CNS) in which we find CTCF plays a vital role. Hi-C experiments on dissected larval CNSs of wildtype controls and CTCF[0] mutants that completely lack CTCF revealed that CTCF forms hundreds of physical boundaries in fly chromosomes.
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| Overall design |
60 wildtype (WT, corresponding to the Drosophila w[1118] reference strain) and CTCF[0] mutant third instar larval CNSs were dissected in biological triplicate and Hi-C libraries were prepared. Hi-C libraries were also prepared from single whole-bodied female flies that were WT or CTCF[0] mutant.
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| Contributor(s) |
Kaushal A, Mohana G, Dorier J, Özdemir I, Omer A, Taschner M, Cousin P, Dergai O, Semenova A, Marzetta F, Iseli C, Eliaz Y, Guex N, Aiden EL, Gambetta MC |
| Citation(s) |
33579945 |
| |
| Submission date |
Mar 10, 2020 |
| Last update date |
Feb 14, 2021 |
| Contact name |
Maria Cristina Gambetta |
| E-mail(s) |
mariacristina.gambetta@unil.ch
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| Organization name |
University of Lausanne
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| Department |
Center for Integrative Genomics
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| Street address |
Genopode Building
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| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
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| Platforms (2) |
| GPL21306 |
Illumina HiSeq 4000 (Drosophila melanogaster) |
| GPL23702 |
HiSeq X Ten (Drosophila melanogaster) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE146752 |
CTCF is required to form regulatory and physical boundaries at limited sites in the Drosophila nervous system |
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| Relations |
| BioProject |
PRJNA611808 |
| SRA |
SRP252247 |
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