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Series GSE146619 Query DataSets for GSE146619
Status Public on Mar 31, 2020
Title Single-cell transcriptomic analysis of adult mouse pituitary reveals sexual dimorphism and physiologic demand-induced cellular plasticity
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The anterior pituitary gland drives a set of highly conserved physiologic processes in mammalian species. These hormonally controlled processes are central to somatic growth, pubertal transformation, fertility, lactation, and metabolism. Current models, largely based on candidate gene based immuno-histochemical and mRNA analyses, suggest that each of the seven hormones synthesized by the pituitary is produced by a specific and exclusive cell-lineage. However, emerging evidence suggests more complex relationship between hormone specificity and cell plasticity. Here we have applied massively parallel single-cell RNA sequencing (scRNA-seq), in conjunction with complementary imaging-based single-cell analyses of mRNAs and proteins, to systematically map both cell-type diversity and functional state heterogeneity in adult male and female mouse pituitaries at single-cell resolution and in the context of major physiologic demands. These quantitative single-cell analyses reveal sex-specific cell-type composition under normal pituitary homeostasis, identify an array of cells associated with complex complements of hormone-enrichment, and undercover non-hormone producing interstitial and supporting cell-types. Interestingly, we also identified a Pou1f1-expressing cell population that is characterized by a unique multi-hormone gene expression profile. In response to two well-defined physiologic stresses, dynamic shifts in cellular diversity and transcriptome profiles were observed for major hormone producing and the putative multi-hormone cells. These studies reveal unanticipated cellular complexity and plasticity in adult pituitary, and provide a rich resource for further validating and expanding our molecular understanding of pituitary gene expression programs and hormone production.
Overall design Single-cell RNA sequencing analysis of adult mouse pituitary under different well-defined physiologic stresses
Contributor(s) Ho Y, Hu P, Peel MT, Chen S, Camara PG, Epstein DJ, Wu H, Liebhaber SA
Citation(s) 32193873
Submission date Mar 09, 2020
Last update date Apr 03, 2020
Contact name Peng Hu
Organization name University of Pennsylvanian
Department Genetics
Lab Hao Wu
Street address 415 Curie Blvd, 545 CRB
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (10)
GSM4399743 WT-8wk-female
GSM4399744 WT-8wk-male
GSM4399745 transgenic-8wk-female
BioProject PRJNA611520
SRA SRP252040

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Supplementary file Size Download File type/resource
GSE146619_RAW.tar 52.9 Mb (http)(custom) TAR (of MTX, TSV, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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