Gene expression profiling in dorsolateral prostates of prepubertal and adult Sprague-Dawley rats dosed with Estradiol Benzoate, Estradiol and Testosterone
Using rat prostate disease models that Prins and colleagues have developed (Ho et al., 2006 - PMID: 16740699), the purpose of the study is to identify gene(s) for diagnosing early stage of prostate diseases and monitoring the development of prostate diseases. To identify the genes, we analyzed whole genome expression profiling on the prostate from Sprague-Dawley rats dosed postnatally with estradiol benzoate, estradiol and testosterone at PNDs 30, 100 and 145.
Overall design
Sprague-Dawley male offspring were injected with 2.5 mg/kg body weight (BW) Estradiol Benzoate (EB) subcutaneously at PNDs 1, 3 and 5 (EB-treated group). Male pups in the untreated (control) group were injected with vehicle. At PND 90, each group (the untreated or EB-treated group) were divided into two additional groups without or with additional hormone treatment [estradiol (E) and testosterone (T)]: control, T+E only, EB only and EB+T+E groups. Animals in the control and EB-only groups were implanted with three empty silastic tubing inserts, while animals in T+E only and EB+T+E groups were implanted with two silastic tubing inserts with T and one silastic tube insert with E. Prostates from PNDs 30, 100 and 145 male Sprague-Dawley rats dosed postnatally with EB, E and T were collected (n=3-4 per group). RNA were extracted and used for whole genome expression array (Agilent Technologies; 8 x 60K format).
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