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Series GSE145917 Query DataSets for GSE145917
Status Public on Aug 17, 2020
Title Gene expression profiling in dorsolateral prostates of prepubertal and adult Sprague-Dawley rats dosed with Estradiol Benzoate, Estradiol and Testosterone
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Using rat prostate disease models that Prins and colleagues have developed (Ho et al., 2006 - PMID: 16740699), the purpose of the study is to identify gene(s) for diagnosing early stage of prostate diseases and monitoring the development of prostate diseases. To identify the genes, we analyzed whole genome expression profiling on the prostate from Sprague-Dawley rats dosed postnatally with estradiol benzoate, estradiol and testosterone at PNDs 30, 100 and 145.
 
Overall design Sprague-Dawley male offspring were injected with 2.5 mg/kg body weight (BW) Estradiol Benzoate (EB) subcutaneously at PNDs 1, 3 and 5 (EB-treated group). Male pups in the untreated (control) group were injected with vehicle. At PND 90, each group (the untreated or EB-treated group) were divided into two additional groups without or with additional hormone treatment [estradiol (E) and testosterone (T)]: control, T+E only, EB only and EB+T+E groups. Animals in the control and EB-only groups were implanted with three empty silastic tubing inserts, while animals in T+E only and EB+T+E groups were implanted with two silastic tubing inserts with T and one silastic tube insert with E. Prostates from PNDs 30, 100 and 145 male Sprague-Dawley rats dosed postnatally with EB, E and T were collected (n=3-4 per group). RNA were extracted and used for whole genome expression array (Agilent Technologies; 8 x 60K format).
 
Contributor(s) Vijay V, Han T, Nakamura N
Citation(s) 32741896
Submission date Feb 25, 2020
Last update date Nov 16, 2020
Contact name Vikrant Vijay
E-mail(s) vikrant.vijay@fda.hhs.gov
Phone 870-543-7525
Organization name US FDA National Center for Toxicological Research
Department Division of Systems Biology
Lab Personalized Medicine
Street address 3900 NCTR Road
City Jefferson
State/province AR
ZIP/Postal code 72079
Country USA
 
Platforms (1)
GPL22740 Agilent-074036 SurePrint G3 Rat GE v2 8x60K Microarray G4858A (Probe Name version)
Samples (33)
GSM4339289 Prostate_PND 30 Male_Control_Rep1
GSM4339290 Prostate_PND 30 Male_Control_Rep2
GSM4339291 Prostate_PND 30 Male_Control_Rep3
Relations
BioProject PRJNA608728

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE145917_RAW.tar 403.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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