NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE14589 Query DataSets for GSE14589
Status Public on Dec 17, 2009
Title A novel hybrid kinase is essential for regulating the σB-mediated stress response of Bacillus cereus.
Organism Bacillus cereus ATCC 14579
Experiment type Expression profiling by array
Summary A common bacterial strategy for monitoring environmental challenges is to use two-component systems, which consist of a sensor histidine kinase (HK) and a response regulator (RR). In the food-borne pathogen Bacillus cereus, the alternative sigma factor σB is activated by the RR RsbY. Here we present strong indications that the PP2C-type phosphatase RsbY receives its input from the multi-sensor hybrid kinase BC1008 (renamed RsbK). Genome analyses revealed that, across bacilli, rsbY and rsbK are located in a conserved gene cluster. A B. cereus rsbK deletion strain was shown to be incapable of inducing σB upon stress conditions and was impaired in its heat adaptive response. Comparison of the wild-type and rsbK mutant transcriptomes upon heat shock revealed that RsbK was primarily involved in the activation of the σB-mediated stress response. Truncation of the RsbK RR receiver domain demonstrated the importance of
this domain for σB induction upon stress. The domain architecture of RsbK suggests that in the B. cereus group and in other bacilli, environmental and intracellular stress signalling routes are combined into one single protein. This strategy is markedly different from the σB activation pathway in other low-GC Grampositives.
 
Overall design Two different comparisons were performed (both in duplo and Cy5-Cy3 dye-swapped):
(1) B. cereus WT 30°C versus B. cereus WT 42°C (10 min heat shock)
(2) B. cereus WT 42°C versus B. cereus FM1404 42°C

Data from comparisons (2) were subsequently compared with transcriptome data obtained previously by Van Schaik et al., 2007
 
Contributor(s) de Been M, Tempelaars MH, van Schaik W, Moezelaar R, Siezen RJ, Abee T
Citation(s) 19958380
Submission date Jan 27, 2009
Last update date Mar 20, 2012
Contact name Mark de Been
Organization name Wageningen UR
Department Agrotechnology and Food Sciences Group
Lab Laboratory of Food Microbiology
Street address Bomenweg 2
City Wageningen
ZIP/Postal code 6703 HD
Country Netherlands
 
Platforms (1)
GPL8114 Bacillus cereus ATCC 14579 - design 3
Samples (4)
GSM364859 WT42 vs WT30
GSM364861 WT30 vs WT42
GSM364865 WT42 vs DrsbK42
Relations
BioProject PRJNA111709

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap