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Series GSE145670 Query DataSets for GSE145670
Status Public on Apr 15, 2020
Title Neuronal upregulation of Prospero protein is driven by alternative mRNA polyadenylation and Syncrip-mediated mRNA stabilisation
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Summary During Drosophila and vertebrate brain development, the conserved transcription factor Prospero/Prox1 is an important regulator of the transition between proliferation and differentiation. Prospero level is low in neural stem cells and their immediate progeny, but is upregulated in larval neurons and it is unknown how this process is controlled. Here, we use single molecule fluorescent in situ hybridisation to show that larval neurons selectively transcribe a long prospero mRNA isoform containing a 15 kb 3’ untranslated region, which is bound in the brain by the conserved RNA-binding protein Syncrip/hnRNPQ. Syncrip binding increases the mRNA stability of the long prosperoisoform, which allows an upregulation of Prospero protein production. Our findings highlight a regulatory strategy involving alternative polyadenylation followed by differential post-transcriptional regulation.
 
Overall design RNA-seq in Drosophila L3 larval brain.
 
Contributor(s) Samuels TJ, Järvelin AI, Davis I
Citation(s) 32205310
Submission date Feb 21, 2020
Last update date May 19, 2020
Contact name Aino Inkeri Järvelin
Organization name University of Oxford
Department Department of Biochemistry
Street address South Parks Road
City Oxford
ZIP/Postal code OX1 3QU
Country United Kingdom
 
Platforms (1)
GPL19528 Ion Torrent Proton (Drosophila melanogaster)
Samples (6)
GSM4330925 OrR RNAseq 1
GSM4330926 OrR RNAseq 2
GSM4330927 OrR RNAseq 3
Relations
BioProject PRJNA607924
SRA SRP250266

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE145670_RAW.tar 407.7 Mb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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