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Series GSE145264 Query DataSets for GSE145264
Status Public on Sep 23, 2020
Title p63 Maintains the Proliferative Potential of Salivary Gland Stem/Progenitor Cells by Regulating Follistatin, a Key Mediator of TGF-b/Activin Signaling [ChIP-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Np63+ve cells are multipotent and maintain all epithelial cell lineages of the embryonic and adult salivary gland (SG). However, the molecular mechanisms by which Np63 regulates stem/progenitor (SP) cell populations in the SG remains elusive. To better understand Np63 s role in directing cell fate choices, here we have utilized Np63-null adult mice and primary salivary cell cultures to probe alterations in SP cell differentiation and function. Specifically, we have generated bulk RNA-seq and scRNA-seq data from Np63-null adult mice and p63 and H3K27Ac ChIP-seq data from primary salivary cell cultures. These genomic and epigenomic data sets were leveraged to interrogate altered SG cellular identities and differentiation states resulting from the loss of Np63. Our studies reveal that ablation of Np63 results in a loss of the SP cell population and skewed SG differentiation that is modulated by dysregulated TGF- /Activin signaling. Our findings offer new molecular revelations into the SP cell gene regulatory networks that are likely to be relevant for normal or diseased SG states.
 
Overall design Analysis of p63 Binding and Histone H3K27ac modification analysis of primary mouse salivary epithelial progenitor cellsĀ using high throughput sequencing
 
Contributor(s) Min S, Oyelakin A, Gluck C, Bard J, Song C, Kirsten S, Che M, Flores E, Sinha S, Romano R
Citation(s) 32932139
https://doi.org/10.1016/j.isci.2020.101524
Submission date Feb 13, 2020
Last update date Oct 02, 2020
Contact name Jonathan E Bard
E-mail(s) jbard@buffalo.edu
Organization name University at Buffalo
Department Genomics and Bioinformatics Core
Street address 701 Ellicott Street
City Buffalo
State/province New York
ZIP/Postal code 14203
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (4)
GSM4310355 PSECS_4A4
GSM4310356 PSECS_4A4 Input
GSM4310357 pSG_H3K27Ac
This SubSeries is part of SuperSeries:
GSE145268 p63 Maintains the Proliferative Potential of Salivary Gland Stem/Progenitor Cells by Regulating Follistatin, a Key Mediator of TGF-b/Activin Signaling
Relations
BioProject PRJNA606548
SRA SRP249337

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Supplementary file Size Download File type/resource
GSE145264_PSECS_4A4_vs_Input.bed.gz 35.2 Kb (ftp)(http) BED
GSE145264_pSG_H3K27Ac_vs_Input.bed.gz 1.5 Mb (ftp)(http) BED
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Raw data are available in SRA
Processed data are available on Series record

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