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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 13, 2020 |
| Title |
In vivo high-throughput screening of novel adeno-associated viral capsids identifies variants for transduction of adult neural stem cells within the subventricular zone |
| Organism |
Mus musculus |
| Experiment type |
Other
Expression profiling by high throughput sequencing
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| Summary |
The adult mammalian brain entails a reservoir of neural stem cells (NSCs) generating glial cells and neurons. However, NSCs become increasingly quiescent with age, which hampers their regenerative capacity. New means are therefore required to genetically modify adult NSCs for re-enabling endogenous brain repair. Recombinant adeno-associated viruses (AAVs) are ideal gene therapy vectors due to an excellent safety profile and high transduction efficiency. We thus conducted a high-throughput screening of 157 intraventricularly injected barcoded AAV variants profiled by transcriptome sequencing. Transcriptome analysis identified two synthetic capsids, AAV9_A2 and AAV1_P5 transducing active and quiescent NSCs. Further optimization of AAV1_P5 by judicious selection of promoter and dose of injected viral genomes enabled targeting of 57% of the NSC compartment. Importantly, transduced NSC readily produced neurons. The present study identifies AAV variants with a high specificity and transduction efficiency of adult NSCs thereby paving the way for preclinical testing of regenerative gene therapy.
We identified a synthetic Adeno-associated virus (AAV) capsid, AAV1_P5, that transduces active and quiescent neural stem cells (NSCs). In order to fully characterize the identity of AAV1_P5-transduced cells, as well as potential changes arising by the AAV-transduction itself, we profiled these cells and untransduced ones from the same mouse by single cell RNA sequencing. To this end, three months-old eYFP-reporter mice were injected with 109 vg/mouse AAV1_P5 harboring the CMV_Cre construct. Transduction induces expression of eYFP. 37 days post injection, we isolated cells from the v-SVZ and other brain regions. More precisely, we isolated labeled cells of the ventricular-subventricular zone and the striatum, rostral migratory stream (RMS) and olfactory bulb, here referred to as rest of the brain (RoB). To capture the remaining unlabeled cells of the NSC-lineage in the v-SVZ, we also isolated GLAST+ v-SVZ cells. Two samples of two pooled mice each were subjected to single cell RNA sequencing.
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| Overall design |
identification of AAV variants with a high specificity and transduction efficiency of adult NSCs
Single cell RNA sequencing of eYFP-reporter brain cells transduced with the synthetic adeno-associated virus capsid AAV1_P5
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| Contributor(s) |
Dehler S, Kremer LP, Stiehl T, Weinmann J, Abendroth H, Laure A, Marciniak-Czochra A, Grimm D, Martin-Villalba A |
| Citation missing |
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| Submission date |
Feb 12, 2020 |
| Last update date |
Aug 22, 2020 |
| Contact name |
Ana Martin-Villalba |
| Organization name |
German Cancer Research Center
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| Department |
Molecular Neurobiology
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| Street address |
Im Neuenheimer Feld 280
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| City |
Heidelberg |
| ZIP/Postal code |
69121 |
| Country |
Germany |
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| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA606261 |
| SRA |
SRP248439 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE145172_RAW.tar |
160.0 Kb |
(http)(custom) |
TAR (of CSV) |
| GSE145172_colNames_genes.tsv.gz |
248.0 Kb |
(ftp)(http) |
TSV |
| GSE145172_metadata.tsv.gz |
187.5 Kb |
(ftp)(http) |
TSV |
| GSE145172_raw_counts_cellXgene.mtx.gz |
41.1 Mb |
(ftp)(http) |
MTX |
| GSE145172_rowNames_cellBarcodes.tsv.gz |
21.6 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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