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Series GSE14491 Query DataSets for GSE14491
Status Public on Apr 03, 2009
Title TGFβ/mutant-p53 jointly controlled genes
Organism Homo sapiens
Experiment type Expression profiling by array
Summary TGFβ ligands act as tumor suppressors in early stage tumors but are paradoxically diverted into potent prometastatic factors in advanced cancers. The molecular nature of this switch remains enigmatic. We now show that TGFβ-dependent cell migration, invasion and metastasis are empowered by mutant-p53.
To investigate the specific gene expression program by which mutant-p53 and TGFβ control invasion and metastasis in breast cancer cells, we compared the TGFβ transcriptomic profile of control and mutant-p53 depleted MDA-MB-231 cells.

Keywords: expression profiling by array
Overall design MDA-MB-231 cells, stably expressing either control (shGFP) or anti-p53 (shp53) short-hairpin RNAs, were left untreated or treated with TGFbeta. Samples were then processed for total RNA extraction and hybridization on Affymetrix microarrays. Four biological replicas (A, B, C, D) were used for each of the 4 conditions (1: untreated control; 2: TGFbeta treated control; 3: untreated p53-depleted cells; 4: TGFbeta treated mutant-p53-depleted cells), for a total of 16 samples.
Contributor(s) Adorno M, Cordenonsi M, Montagner M, Dupont S, Wong C, Hann B, Solari A, Bobisse S, Rondina M, Guzzardo E, Parenti A, Rosato A, Bicciato S, Balmain A, Piccolo S
Citation(s) 19345189
Submission date Jan 21, 2009
Last update date Jan 08, 2019
Contact name Silvio Bicciato
Phone +39-059-205-5219
Organization name University of Modena and Reggio Emilia
Department Life Sciences
Lab Center for Genome Research
Street address via Campi, 287
City Modena
ZIP/Postal code 41125
Country Italy
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (16)
GSM361954 MDA shGFP, untreated, biological replicate A
GSM361955 MDA shGFP, untreated, biological replicate B
GSM361956 MDA shGFP, untreated, biological replicate C
BioProject PRJNA111547

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Supplementary file Size Download File type/resource
GSE14491_RAW.tar 71.2 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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