|
| Status |
Public on May 07, 2020 |
| Title |
OppZ target identification |
| Organism |
Vibrio cholerae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study we determined the target spectrum of the Vibrio cholerae OppZ sRNA via pulse-expression followed by RNA-sequencing.
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| |
|
| Overall design |
V. cholerae wild-type cells carrying pBAD1K-ctrl (empty control vector) or pBAD1K-oppZ (inducible sRNA expression vector) were grown to exponential phase (OD600=0.5). Expression of OppZ was induced by L-arabinose and total RNA samples were collected 15 minutes after induction. The experiment was performed with three independent biological replicates.
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| |
|
| Contributor(s) |
Hoyos M, Huber M, Förstner K, Papenfort K |
| Citation(s) |
32744240 |
| |
| Submission date |
Jan 29, 2020 |
| Last update date |
Aug 06, 2020 |
| Contact name |
Mona Hoyos |
| Organization name |
FSU Jena
|
| Department |
Institute for General Microbiology
|
| Lab |
Kai Papenfort
|
| Street address |
Winzerlaer Straße 2
|
| City |
Jena |
| ZIP/Postal code |
07745 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL25180 |
Illumina HiSeq 1500 (Vibrio cholerae) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA603761 |
| SRA |
SRP245878 |
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