|
| Status |
Public on May 07, 2020 |
| Title |
Identification of bicyclomycin-sensitive transcripts in Vibrio cholerae |
| Organism |
Vibrio cholerae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study we used BCM, inhibitor of Rho activity, to determine Rho-dependent transcription termination sites in V. cholerae.
|
| |
|
| Overall design |
V. cholerae oppA::3xFLAG oppB::3xFLAG strains with wild-type oppB or the oppB start codon mutated to ATC were grown to early stationary phase (OD600 of 1.5). Cultures were divided in half and treated with either H2O (mock treatment) or BCM (25 µg/ml final conc.) for 2h before RNA samples were collected. The experiment was performed with three independent biological replicates.
|
| |
|
| Contributor(s) |
Hoyos M, Huber M, Förstner K, Papenfort K |
| Citation(s) |
32744240, 36482060 |
| |
| Submission date |
Jan 29, 2020 |
| Last update date |
Jan 04, 2023 |
| Contact name |
Mona Hoyos |
| Organization name |
FSU Jena
|
| Department |
Institute for General Microbiology
|
| Lab |
Kai Papenfort
|
| Street address |
Winzerlaer Straße 2
|
| City |
Jena |
| ZIP/Postal code |
07745 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL25005 |
Illumina NextSeq 500 (Vibrio cholerae) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA603762 |
| SRA |
SRP245877 |
Less...
More...