|
| Status |
Public on Jun 22, 2020 |
| Title |
Single Cell and Spatial Analysis of Human Squamous Cell Carcinoma [CRISPR screen] |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
To define the cellular composition and spatial architecture of the tumor micoenvironment, we combined single-cell RNA-sequencing, spatial transcriptomics, and multiplexed ion-beam imaging from 10 patient cutaneous squamous cell carcinoma tumors and site-matched normal skin
|
| |
|
| Overall design |
SCC cell lines were infected with a CRISPR sgRNA library of 334 genes (and 136 non-targeting sgRNA controls) and grown either as xenograft tumors in immunocomprised mice or in vitro, followed by sequencing of sgRNAs
|
| |
|
| Contributor(s) |
Ji AL, Rubin AJ |
| Citation(s) |
32579974 |
| |
| Submission date |
Jan 24, 2020 |
| Last update date |
May 02, 2022 |
| Contact name |
Andrew Ji |
| E-mail(s) |
andrew.ji@mssm.edu
|
| Organization name |
Icahn School of Medicine at Mount Sinai
|
| Street address |
1428 Madison Ave.
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
|
| Platforms (1) |
|
| Samples (48)
|
|
| This SubSeries is part of SuperSeries: |
| GSE144240 |
Single Cell and Spatial Analysis of Human Squamous Cell Carcinoma |
|
| Relations |
| BioProject |
PRJNA603105 |
| SRA |
SRP244707 |
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