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| Status |
Public on Jan 06, 2021 |
| Title |
Identification of Pol II(G) target genes in mouse liver [ChIP-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To investigate the biological role of Pol II(G), we identified Pol II(G) target genes in normal mouse liver.
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| Overall design |
To identify genes that are directly targeted by Pol II(G) in hepatocytes, we performed ChIP-seq analysis with non-crosslinked chromatin prepared from Gdown1 flox/flox (FF) or Gdown1flox/flox;Alb-Cre (KO) liver at 7W of age. We also analyzed Gdown1 target genes in p53KO hepatocytes. For the negative control, tamoxifen (TAM)-inducible Gdown1flox/floxKO mice carrying both the Alb-CreERT2 transgene and a p53 null mutation was used.
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| Contributor(s) |
Jishage M |
| Citation(s) |
32381628 |
| |
| Submission date |
Jan 24, 2020 |
| Last update date |
Jan 06, 2021 |
| Contact name |
Miki JIshage |
| E-mail(s) |
jishagm@mail.rockefeller.edu
|
| Phone |
212-327-7605
|
| Organization name |
Rockefeller University
|
| Street address |
1230 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE144212 |
Transcriptional down-regulation of metabolic genes by Gdown1 ablation induces quiescent cell re-entry into the cell cycle |
|
| Relations |
| BioProject |
PRJNA603065 |
| SRA |
SRP244621 |
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