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| Status |
Public on Jan 06, 2021 |
| Title |
Differentially expressed genes in Gdown1 knockout and Gdown1/p53 double-knockout mouse liver [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate the biological role of Gdown1, differentially expressed genes in Gdown1 knockout and p53/Gdown1 double-knockout mouse liver were analyzed by RNA-seq.
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| Overall design |
To investigate differentially expressed genes in Gdown1 knockout (KO) hepatocytes in the presence or absence of p53, total RNA was isolated from liver tissues of the following genotypes: Gdown1 flox/flox (FF), Gdown1flox/flox;Alb-Cre (KO), Gdown1 flox/flox;p53-/- (p53KO), and Gdown1flox/flox;Alb-Cre;p53-/- (DKO). To investigate the immediate effects of the Gdown1 KO, we generated tamoxifen (TAM)-inducible Gdown1flox/floxKO mice carrying both the Alb-CreERT2 transgene and a p53 null mutation (DKOAlb-CreERT2mice). The liver tissues were collected from p53KO or DKOAlb-CreERT2 mice at 24 hours after tamoxifen injection.
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| Contributor(s) |
Jishage M |
| Citation(s) |
32381628 |
| |
| Submission date |
Jan 24, 2020 |
| Last update date |
Jan 06, 2021 |
| Contact name |
Miki JIshage |
| E-mail(s) |
jishagm@mail.rockefeller.edu
|
| Phone |
212-327-7605
|
| Organization name |
Rockefeller University
|
| Street address |
1230 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE144212 |
Transcriptional down-regulation of metabolic genes by Gdown1 ablation induces quiescent cell re-entry into the cell cycle |
|
| Relations |
| BioProject |
PRJNA603066 |
| SRA |
SRP244620 |
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