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| Status |
Public on Jan 27, 2021 |
| Title |
Cellular and molecular characterization of the dural sinuses as a neuroimmune interface [vasculature] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Meningeal immunity is critical to appropriate CNS function. Here we perform single cell RNA-sequencing to investigate how brain and meningeal stromal populations regulate immune trafficking and retention in young and old mice.
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| Overall design |
15 young (2 months) and 15 old (24 months) mice were sacrificed with euthasol and brain and dural meninges were collected. Tissues were dissociated with collagenase 8 to obtain a single cell suspension and sorted by FACS based on the following phenotypes: endothelial DAPI-Singlets CD45-CD31+CD13- and mural DAPI-Singlets CD45-CD31-CD13+. Equal amounts of endothelial and mural populations were pooled for single cell sequencing.
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| Contributor(s) |
Rustenhoven J, Dykstra T, Kipnis J |
| Citation(s) |
33508229 |
| |
| Submission date |
Jan 23, 2020 |
| Last update date |
May 24, 2021 |
| Contact name |
Jonathan Kipnis |
| Organization name |
Washington University in Saint Louis
|
| Department |
Pathology and Immunology
|
| Lab |
Kipnis Lab
|
| Street address |
4515 McKinley Ave
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (12)
|
| GSM4282417 |
Meninges, Young |
| GSM4904360 |
Brain, old, CD31+ |
| GSM4904361 |
Brain, old, CD13+ |
| GSM4904362 |
Brain, young, CD31+ |
| GSM4904363 |
Brain, young, CD13+ |
| GSM4904364 |
Meninges, old, CD31+ |
| GSM4904365 |
Meninges, old, CD13+ |
| GSM4904366 |
Meninges, young, CD31+ |
| GSM4904367 |
Meninges, young, CD13+ |
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| This SubSeries is part of SuperSeries: |
| GSE144175 |
Cellular and molecular characterization of the dural sinuses as a neuroimmune interface |
|
| Relations |
| BioProject |
PRJNA602933 |
| SRA |
SRP244448 |
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