NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE144115 Query DataSets for GSE144115
Status Public on Aug 10, 2020
Title Distinct and overlapping roles of STAG1 and STAG2 in cohesin localization and gene expression in embryonic stem cells [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: Cohesin is a ring-shaped complex that is critical to genome organization and is an important regulator of gene expression. How cohesin accessory proteins contribute to cohesin function remain unclear. The purpose of this study is to assess the roles of cohesin accessory proteins, STAG1 and STAG2, in cohesin localization and gene expression.

Method: The role of STAG1 and STAG2 in gene regulation was assessed by performing RNA-seq in wildtype and CRISPR-Cas9 genome edited STAG knockout mouse embryonic stem cells (mESCs). The redundancy of the STAGs was addressed by using siRNA against STAG1 in a STAG2-knockout background.
 
Overall design RNA-seq was performed in wild type mESCs, STAG-knockout CRISPR-Cas9 genome edited mESCs, and STAG-knockout mESCs with siRNA depletion of the other STAG, all in triplicate.
 
Contributor(s) Dowen JM, Arruda NL, Carico ZM, Justice M, Liu YF, Zhou J, Stefan HC
Citation(s) 32778134
Submission date Jan 23, 2020
Last update date Mar 29, 2022
Contact name Jill Dowen
E-mail(s) jilldowen@unc.edu
Organization name University of North Carolina at Chapel Hill
Street address 250 Bell Tower Drive
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (15)
GSM4280519 WT siGLO Rep 1
GSM4280520 WT siGLO Rep 2
GSM4280521 WT siGLO Rep 3
This SubSeries is part of SuperSeries:
GSE144116 Distinct and overlapping roles of STAG1 and STAG2 in cohesin localization and gene expression in embryonic stem cells
Relations
BioProject PRJNA602822
SRA SRP244261

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE144115_RAW.tar 1.5 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap