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| Status |
Public on Aug 10, 2020 |
| Title |
Distinct and overlapping roles of STAG1 and STAG2 in cohesin localization and gene expression in embryonic stem cells [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: Cohesin is a ring-shaped complex that is critical to genome organization and is an important regulator of gene expression. How cohesin accessory proteins contribute to cohesin function remain unclear. The purpose of this study is to assess the roles of cohesin accessory proteins, STAG1 and STAG2, in cohesin localization and gene expression.
Method: The role of STAG1 and STAG2 in gene regulation was assessed by performing RNA-seq in wildtype and CRISPR-Cas9 genome edited STAG knockout mouse embryonic stem cells (mESCs). The redundancy of the STAGs was addressed by using siRNA against STAG1 in a STAG2-knockout background.
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| Overall design |
RNA-seq was performed in wild type mESCs, STAG-knockout CRISPR-Cas9 genome edited mESCs, and STAG-knockout mESCs with siRNA depletion of the other STAG, all in triplicate.
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| Contributor(s) |
Dowen JM, Arruda NL, Carico ZM, Justice M, Liu YF, Zhou J, Stefan HC |
| Citation(s) |
32778134 |
| |
| Submission date |
Jan 23, 2020 |
| Last update date |
Mar 29, 2022 |
| Contact name |
Jill Dowen |
| E-mail(s) |
jilldowen@unc.edu
|
| Organization name |
University of North Carolina at Chapel Hill
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| Street address |
250 Bell Tower Drive
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE144116 |
Distinct and overlapping roles of STAG1 and STAG2 in cohesin localization and gene expression in embryonic stem cells |
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| Relations |
| BioProject |
PRJNA602822 |
| SRA |
SRP244261 |
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