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Series GSE14406 Query DataSets for GSE14406
Status Public on Jan 31, 2009
Title Oligodendroglial precursor cell line [Oli-neu] undergoing differentiation into myelin basic protein-producing cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Inadequate remyelination of brain white matter lesions has been associated with a failure of oligodendrocyte precursors to differentiate into mature, myelin-producing cells. In order to better understand which genes play a specific role in oligodendrocyte differentiation we performed time dependent, genome-wide gene expression studies of mouse Oli-neu cells as they differentiate into myelin basic protein-producing cells, following treatment with three different agents. Our data indicate that different inducers activate distinct pathways that ultimately converge into the differentiated state where regulated gene sets overlap maximally.
In order to also gain insight into the functional role of genes that are regulated in this process, we silenced 88 of these genes using siRNA, and identified multiple repressors of spontaneous differentiation of Oli-neu, most of which were confirmed in rat primary oligodendrocyte precursors cells. Among these repressors were CNPase, a well-known myelin constituent, and three phosphatases, each known to negatively control MAP kinase cascades. We show that a novel inhibitor for one of the identified genes, dual-specificity phosphatase DUSP10/MKP5, was also capable of inducing oligodendrocyte differentiation in primary oligodendrocyte precursors. Oligodendrocytic differentiation feedback loops may therefore yield pharmacological targets to treat disease related to dysfunctional myelin deposition

Keywords: time course
 
Overall design triplicates for 3 times points 10h, 24h, 72h in 6 conditions, Forskolin, Insulin, Dexamethasone, Retinoic Acid, PD174265, Untreated. Arrays were done in two distinct experiments 1 and 2. Some replicates are missing because of lab operating issues
 
Contributor(s) Gobert RP, Joubert L, Curchod M, Salvat C, Foucault I, Jorand-Lebrun C, Lamarine M, Peixoto H, Vignaud C, Frémaux C, se Jomotte T, Françon B, Alliod C, Bernasconi L, Abderrahim H, Perrin D, Bombrun A, Zanoguera F, Rommel C, van Huijsduijnen RH
Citation(s) 19139271
Submission date Jan 13, 2009
Last update date Feb 11, 2019
Contact name Marc Lamarine
E-mail marc.lamarine@merckserono.net
Phone +41 22 414 9175
Fax +41 22 414 9558
Organization name Merck Serono S.A. · Geneva
Street address 9 Chemin des Mines
City Geneva
State/province Geneva
ZIP/Postal code 1202
Country Switzerland
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (54)
GSM360951 Dexamethasone at 10 h, biol. rep1 in Exp1
GSM360952 Dexamethasone at 10 h, biol. rep2 in Exp1
GSM360953 Dexamethasone at 10 h, biol. rep3 in Exp1
Relations
BioProject PRJNA111797

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE14406_RAW.tar 318.7 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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