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Series GSE143924 Query DataSets for GSE143924
Status Public on Jun 01, 2020
Title Transcriptome analysis from human epicardial adipose tissue biopsies analyzed according to postoperative atrial fibrillation occurrence after cardiac surgery
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Introduction: Post-operative atrial fibrillation (POAF) is a frequent complication after cardiac surgery, but its pathophysiology remains incompletely understood. Considering that epicardial adipose tissue (EAT) is in close vicinity with the atrial myocardium, we hypothesized that a specific pre-operative EAT phenotype would be associated to POAF onset following surgery. Methods: Patients undergoing cardiac surgery prospectively enrolled in the POMI-AF cohort between February 2016 and June 2017 were studied. EAT samples were collected at the beginning of surgery. Whole-tissue gene expression patterns and the stromal and vascular fraction (SVF) cellular composition were explored. Patients were followed after surgery by continuous ECG to detect POAF onset. Results: Among the 60 patients included in the cohort, 15 POAF and 15 non-POAF patients were matched based on pre-operative characteristics. Gene set enrichment analysis of transcriptomic data from pre-operative EAT samples revealed 40 enriched biological processes in POAF vs non-POAF patients. Most of these processes were related to cellular immune response. Leukocytes (63±15% of total cells), and more specifically lymphocytes (56±13% of total CD45+ cells), represented the major cell subset in the preoperative EAT SVF, with no quantitative differences between POAF and SR patients (76 [52; 84]% vs 56 [50; 64]%, p=0.22). However, POAF patients presented a significantly higher cytotoxic CD8+/helper CD4+ T lymphocyte ratio than SR patients (respectively, 0.69[0.55; 1.19] vs 0.50 [0.31; 0.54], p=0.03) suggesting a cytotoxic shift prior to surgery. Conclusion: Epicardial fat from patients who develop POAF displays a specific pre-operative transcriptome signature characteristic of cellular immune response and cytotoxic lymphocyte enrichment.
 
Overall design 30 matched samples analyzed (15 POAF vs 15 SR patients)
 
Contributor(s) Ninni S, Woitrain E, Coisne A, Gheeraert C, Ortmans S, Modine T, Koussa M, Juthier F, Vincentelli A, Molendi-Coste O, Pineau L, Lefebvre P, Dombrowicz D, Staels B, Montaigne D
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Submission date Jan 19, 2020
Last update date Jun 03, 2020
Contact name Sandro Ninni
E-mail(s) sandro.ninni@hotmail.fr
Organization name INSERM
Lab UMR1011
Street address 1 rue du Professeur Calmette
City Lille
ZIP/Postal code 59019
Country France
 
Platforms (1)
GPL25483 [HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [gene symbol version]
Samples (30)
GSM4276706 SR_381_15
GSM4276707 SR_335_5
GSM4276708 SR_391_17
Relations
BioProject PRJNA602162

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE143924_RAW.tar 230.0 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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