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| Status |
Public on Aug 11, 2020 |
| Title |
RNA seq analysis of primary tumors and metastases from the NPK (Nkx3.1CreERT2/+; Ptenflox/flox; KrasLSL-G12D/+; R26R-CAG-LSL-EYFP/+) prostate cancer mouse model |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Transcriptome analysis of lineage-marked prostate primary tumors and metastases from the NPK mouse model (Nkx3.1CreERT2/+; Ptenflox/flox; KrasLSL-G12D/+; R26R-CAG-LSL-EYFP/+)
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| Overall design |
Male mice of a a predominantly C57BL/6 background were induced to form tumors at 2-3 months of age by administration of tamoxifen using 100 mg/kg (in corn oil) once daily for 4 consecutive days. Following tamoxifen-induction, mice were monitored daily and euthanized when their body condition score was <1.5, or when they experienced body weight loss >20% or signs of distress, such as difficulty breathing or bladder obstruction. Transcriptomic analysis of bulk tissues was done on primary tumors (n=19) and matched macrodissected metastases from lung (n=11), liver (n=5), lymph nodes (n=4), or bone (n=12) from 11 independent mice. RNA was prepared from snap-frozen tissues the using MagMAX-96 total RNA isolation kit and only RNA samples having between >200 ng and 1 ug and with an RNA integrity number (RIN) > 8 were used.
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| Contributor(s) |
Arriaga JM, Mitrofanova A, Abate-Shen C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jan 16, 2020 |
| Last update date |
Aug 11, 2020 |
| Contact name |
Antonina Mitrofanova |
| E-mail(s) |
mitrofanova.antonina@hotmail.com
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| Organization name |
Rutgers University
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| Street address |
65 Bergen Street, Rm 923B
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| City |
Newark |
| State/province |
NJ |
| ZIP/Postal code |
07107 |
| Country |
USA |
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| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (62)
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| This SubSeries is part of SuperSeries: |
| GSE143815 |
Primary tumors and metastases from the NPK (Nkx3.1CreERT2/+; Ptenflox/flox; KrasLSL-G12D/+; R26R-CAG-LSL-EYFP/+) prostate cancer mouse model |
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| Relations |
| BioProject |
PRJNA601755 |
| SRA |
SRP242294 |
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