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Series GSE143756 Query DataSets for GSE143756
Status Public on Mar 01, 2020
Title Next Generation Sequencing Facilitates Quantitative Analysis of WT AHCY E14.1 cell and T136A AHCY E14.1 cell
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: To test the effect of AHCY O-GlcNAcylation on E14.1cell pluripotency and The goals of this study are to compare gene expression profiles of the WT AHCY rescue cells and the T136A AHCY rescue cells.
Method:Total RNA was Trizol-extracted and purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in NEB. cDNA was synthesized using random hexamer primer and RNase H. The library fragments were purified with QiaQuick PCR kits and elution with EB buffer, then terminal repair A-tailing and adapter added were implemented. The clustering of the index-coded samples was performed on a cBot cluster generation system using HiSeq PE Cluster Kit v4-cBot-HS (Illumina) according to the manufacturer’s instructions. After cluster generation, the libraries were sequenced on an Illumina platform and 150 bp paired-end reads were generated.
Results:Significant alterations in gene expression levels were observed, with 1351 genes upregulated and 979 genes downregulated in the T136A AHCY rescue cells
Overall design the mRNA profiles of WT AHCY E14.1 cells and T136A AHCY E14.1 cells were generated by deep sequencing using Illumina GAIIx.
Contributor(s) qiang z
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Submission date Jan 15, 2020
Last update date Mar 01, 2020
Contact name qiang zhu
Organization name Zhe Jiang university
Street address yuhangtang road 866
City hang zhou
State/province zhe jiang
ZIP/Postal code 310058
Country China
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (2)
GSM4274225 E14.1WT
GSM4274226 E14.1T136A
BioProject PRJNA601486
SRA SRP242104

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE143756_RAW.tar 430.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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