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Series GSE143688 Query DataSets for GSE143688
Status Public on Apr 23, 2020
Title IL-36 signaling in keratinocytes controls early IL-23 production in Aldara®-induced skin inflammation in mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary IL-36R signaling plays an important role in the pathogenesis of psoriasis. We ought to assess the specific function of IL-36R in keratinocytes for the pathology of Aldara®-induced psoriasis-like skin inflammation. Il36r-/- (KO) and Il36rΔK (DK) mice as well as their littermate controls, respectively Il36r+/+ (WT) and Il36rfl/fl (FL) mice, were challenged with the topical application of Aldara® on one ear during 3 or 7 days. RNASeq was performed on treated (AL) and untreated (CT) ears. Fold change analysis combined with RPKM and RT-qPCR allowed us to identify genes which were specifically regulated by IL-36R signaling in keratinocytes during the course of the treatment. We notably demonstrated that both IL-23 subunits (Il23a and Il12b) fell into this category.
 
Overall design Skin mRNA profiles from WT, KO, FL and DK mice in AL and CT ears at days 3 and 7 of Aldara treatment (4-6 replicates per condition) were generated using TruSeq mRNA stranded kit on an Illumina HiSeq 4000 sequencer.
 
Contributor(s) Goldstein JD, Caruso A, Lemeille S, Gabay C
Citation(s) 32345660
Submission date Jan 14, 2020
Last update date Aug 03, 2020
Contact name Sylvain LEMEILLE
Organization name University of Geneva Medical School
Department Department of Pathology and Immunology
Street address 1 rue Michel-Servet
City Geneva
ZIP/Postal code 1211
Country Switzerland
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (74)
GSM4272588 d3 DK AL rep1
GSM4272589 d3 DK AL rep2
GSM4272590 d3 DK AL rep3
Relations
BioProject PRJNA601253
SRA SRP241978

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Supplementary file Size Download File type/resource
GSE143688_Raw_counts.txt.gz 2.8 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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