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| Status |
Public on Jan 06, 2021 |
| Title |
Altered Innate Immunity Confers Staphylococcus aureus Resistance in O-glycosylation Deficient Caenorhabditis elegans bus Mutants |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
|
| Summary |
The Caenorhabditis elegans bus (bacterial unswollen) mutants were isolated by their altered response to the nematode pathogen Microbacterium nematophilum. The bus-2, bus-4 and bus-17 mutants are resistant to infection by this bacterium and to infection by human pathogens Yersinia pestis and Yersinia pseudotuberculosis. Here we extend that list to Staphylococcus aureus. The bus-2, bus-4 and bus-17 mutants each harbors a defect in a different glycosyltransferase involved in O-glycosylation. Glycomics analysis of these strains reveals significant O-glycosylation defects. We further investigated the nature of bus mutant phenotypes in bus-2, bus-4 and bus-17 by gene expression analysis. Three distinct areas of altered expression were identified: 1) N- and O-glycosylation; 2) innate immune response; 3) protein folding and editing control. As expected N- and O-glycosylation gene expression was altered at key enzymatic steps. Innate immune system expression patterns were altered in a way that significantly overlapped with expression patterns seen in wild-type upon exposure to Staphylococuss aureus. Upon infection with S. aureus markers of innate immune activity increased significantly compared to wild-type. The abu/pqn genes, active in the non-canonical unfolded protein response (UPR) pathway were dramatically upregulated in bus when these mutants were exposed to the pathogen. This work demonstrates a genetic link between O-glycosylation and expression of key components of the innate immune response.
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| Overall design |
Identification of genes expressed in three groups with 5 groups three replications (N2D as control)
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| Contributor(s) |
Ghosh SK, Mizanur RM, Bond MR, Jankowska E, Hanover JA, Cipollo JF |
| Citation missing |
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| |
| Submission date |
Jan 14, 2020 |
| Last update date |
Jan 07, 2021 |
| Contact name |
WeiPing Chen |
| E-mail(s) |
weipingChen@niddk.nih.gov
|
| Phone |
301-496-0175
|
| Organization name |
NIDDK/NIH
|
| Department |
GCL
|
| Lab |
Genomics Core Lab
|
| Street address |
Bldg 8, Room 1A11, NIDDK/NIH
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
|
| Samples (15)
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| GSM4271801 |
09-19-12_JCipollo4_SG_Celegans_4_bus2_D1 |
| GSM4271802 |
09-19-12_JCipollo5_SG_Celegans_5_bus2_D2 |
| GSM4271803 |
09-19-12_JCipollo6_SG_Celegans_6_bus2_D3 |
| GSM4271804 |
09-19-12_JCipollo7_SG_Celegans_7_bus4_D1 |
| GSM4271805 |
09-19-12_JCipollo8_SG_Celegans_8_bus4_D2 |
| GSM4271806 |
09-19-12_JCipollo9_SG_Celegans_9_bus4_D3 |
| GSM4271807 |
09-19-12_JCipollo10_SG_Celegans_10_bus17_D1 |
| GSM4271808 |
09-19-12_JCipollo11_SG_Celegans_11_bus17_D2 |
| GSM4271809 |
09-19-12_JCipollo12_SG_Celegans_12_bus17_D3 |
| GSM4271810 |
09-19-12_JCipollo13_SG_Celegans_13_srf5_D1 |
| GSM4271811 |
09-19-12_JCipollo14_SG_Celegans_14_srf5_D2 |
| GSM4271812 |
09-19-12_JCipollo15_SG_Celegans_15_srf5_D3 |
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| Relations |
| BioProject |
PRJNA601206 |
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