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| Status |
Public on Aug 31, 2020 |
| Title |
Batf3 programs CD8 T cell memory |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Anti-viral CD8 T cell responses are characterized by an initial activation/priming of T lymphocytes followed by a massive proliferation, subset differentiation, population contraction and the development of a stable memory pool. The transcription factor BATF3 has been shown to play a central role in the development of conventional dendritic cells (cDC1), which in turn are critical for the optimal priming of CD8 T cells. Here we show that BATF3 is expressed within the first days after priming but has long-lasting T cell intrinsic effects. We found that T cells that lack Batf3 show a normal expansion and differentiation, yet succumbed to an aggravated contraction and had a diminished memory response. Vice versa BATF3-overexpression in CD8 T cells promoted their survival and transition to memory. Mechanistically, BATF3 regulates T cell apoptosis and longevity via the proapoptotic factor BIM. By programing CD8 T cell survival and memory, BATF3 is a promising molecule to optimize adoptive T cell therapy in patients.
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| Overall design |
CD8+ T cells were isolated from spleen of TCR transgenic OT-I mice. Cells were transduced either with an empty or Batf3 expressing vector (see the Retroviral transduction M&M session). Successfully transduced CD8 T cells were FACS sorted and cultured for 6h in supplemented medium with IL-15 (100ng/ml) (non-stimulated) or supplemented medium plus anti-CD3/CD28 (1ug/ml) (stimulated). Three technical replicates from each condition were used for the bulk sequencing.
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| Contributor(s) |
Ataide M, Kastenmueller W |
| Citation(s) |
32989328 |
| Submission date |
Jan 13, 2020 |
| Last update date |
Nov 30, 2020 |
| Contact name |
Wolfgang Kastenmüller |
| E-mail(s) |
wolfgang.kastenmueller@uni-wuerzburg.de
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| Organization name |
University of Wuerzburg
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| Street address |
Versbacher Str. 9
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| City |
Würzburg |
| ZIP/Postal code |
97078 |
| Country |
Germany |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA600843 |
| SRA |
SRP241693 |
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