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Series GSE143302 Query DataSets for GSE143302
Status Public on Apr 07, 2021
Title Adenylate kinase 4 Promotes the expression of Inflammatory Genes in Macrophages via Hif-1a
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Macrophages comprise the first line of defense against various pathogens. Classically activated macrophages (M1), induced by IFNg and LPS, express a high level of inflammatory cytokines and contribute to inflammatory processes. By contrast, alternatively activated macrophages (M2) are induced by IL-4/IL-13, produce IL-10, and display anti-inflammatory activity. Adenylate kinase 4 (Αk4), an enzyme that transfers phosphate group among ATP/GTP, AMP, and ADP, is a key modulator of ATP and is involved in maintaining the homeostasis of cellular nucleotides which is essential for the function of cells. However, its role in regulating the function of macrophages is not fully understood. Here we report that the expression of Ak4 is induced in M1 but not M2 macrophages. Suppressing the expression of Ak4 in M1 macrophages with shRNA enhances the production of ATP. RNA-seq analysis identifies several inflammation-related genes, including Il1b, Il6, Tnfa, Nos2 and Hif1a, as downstream targets of Ak4. We further demonstrate that Ak4 and Hif1a form a positive feedback loop in sustaining the expression of the inflammation-related genes. Our data depict a potential mechanism linking energy consumption and inflammation in macrophages.
 
Overall design Total M1 macrophages mRNA profile of scramble and Ak4 shRNA KD
 
Contributor(s) Miaw  
Citation(s) 33790902
Submission date Jan 08, 2020
Last update date Apr 07, 2021
Contact name Shi-Chuen Miaw
E-mail(s) smiaw@ntu.edu.tw
Phone 0939676785
Organization name National Taiwan University, College of Medicine
Department Immunology
Street address No.1 Jen Ai road section 1
City Taipei
ZIP/Postal code 100
Country Taiwan
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (6)
GSM4256677 Scramble M1 rep1
GSM4256678 Scramble M1 rep2
GSM4256679 Scramble M1 rep3
Relations
BioProject PRJNA599952
SRA SRP240358

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE143302_Analysis_Result_version3_20191111.xlsx 10.3 Mb (ftp)(http) XLSX
GSE143302_Raw_count.txt.gz 477.7 Kb (ftp)(http) TXT
GSE143302_normalied.txt.gz 722.8 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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