|
| Status |
Public on Jun 30, 2020 |
| Title |
Ribosome profiling under ABCE1 depletion to study aberrant translation termination |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report the effect of siRNA-mediated ABCE1 KD in HeLa cells, using a modified ribosome profiling protocol adapted from Ingolia et al., 2011 in order to specifically investigate ABCE1 role on translation termination
|
| |
|
| Overall design |
Control and ABCE1 KD conditions, three replicates for condition. totalRNA samples in parallel for input control.
|
| |
|
| Contributor(s) |
Annibaldis G, Dreos R, Muhlemann O |
| Citation(s) |
32807779 |
| |
| Submission date |
Jan 08, 2020 |
| Last update date |
Oct 15, 2020 |
| Contact name |
Rene Dreos |
| E-mail(s) |
rene.dreos@unil.ch
|
| Phone |
0044 (0)1603 450793
|
| Organization name |
UNIL
|
| Department |
CIG
|
| Lab |
Gatfield Lab
|
| Street address |
Ecublens
|
| City |
Lausanne |
| State/province |
VD |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (21)
|
|
| Relations |
| BioProject |
PRJNA599943 |
| SRA |
SRP240357 |
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