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| Status |
Public on Dec 23, 2020 |
| Title |
Whole transcriptome data of human liver organoids cultured in Matrigel and polyisocyanopeptides-based hydrogel |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
End-stage liver diseases are an increasing health burden and liver transplantations are currently the only curative treatment option. Due to a lack of donor livers, alternative treatments are urgently needed. Human liver organoids are very promising for regenerative medicine, however, organoids are currently cultured in Matrigel, which is extracted from the extracellular matrix of the Engelbreth-Holm-Swarm mouse sarcoma. Matrigel is poorly defined, suffers from high batch-to-batch variability and is of murine origin, which limits clinical application of organoids. Here, a novel hydrogel based on polyisocyanopeptides (PIC) and laminin-111 is described for human liver organoid culture. PIC is a synthetic hydrogel with thermodynamic properties, making it easy to handle and very attractive for clinical applications. Organoids in an optimized PIC hydrogel proliferate at rates comparable to Matrigel; proliferation rates are stiffness-dependent, with lower stiffnesses being optimal for organoid proliferation. Moreover, organoids can be efficiently differentiated towards hepatocyte-like cells with key liver functions. This proliferation and differentiation potential can be maintained over at least 16 passages. Our results indicate that PIC is a promising material for human liver organoid culture and has the potential to be used in a variety of clinical applications including cell therapy and tissue engineering.
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| Overall design |
Whole transcriptome data of human liver organoids cultured in Matrigel or a novel hybrid hydrogel based on polyisocyanopeptides (PIC) and laminin-111. Hydrogel stiffness varied by using PIC different molecular weights, namely 1 kDa PIC (1k PIC) and 5 kDa PIC (5k PIC). The proliferative capacity of organoids in hydrogel was tested by culturing in organoid expansion medium (EM). By culturing liver organoids in differentiation medium (DM) these organoids are capable to differentiate towards hepatocyte-like cells. RNA Seq data is produced on Illumina NextSeq500 using a single-end 75 bp configuration.
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| Web link |
https://doi.org/10.1002/adfm.202000893
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| Contributor(s) |
Ye S, Boeter J, Mihajlovic M, van Steenbeek FG, van Wolferen ME, Oosterhoff LA, Marsee A, Caiazzo M, van der Laan LJ, Penning LC, Vermonden T, Spee B, Schneeberger K |
| Citation(s) |
34658689 |
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| Submission date |
Jan 07, 2020 |
| Last update date |
Nov 18, 2021 |
| Contact name |
Frank van Steenbeek |
| E-mail(s) |
f.g.vansteenbeek@uu.nl
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| Phone |
0302532848
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| Organization name |
Utrecht University
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| Street address |
Yalelaan 104
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| City |
Utrecht |
| State/province |
Select State/Province |
| ZIP/Postal code |
3584 CM |
| Country |
Netherlands |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA599343 |
| SRA |
SRP239777 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE143223_MOK4143.txt.gz |
504.8 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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