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Series GSE14305

Query DataSets for GSE14305

Status

Public on Feb 05, 2010

Title

The microstructural, mineralogical and transcriptional developments of shell biomineralization of Pinctada maxima

Organism

Pinctada maxima

Experiment type

Expression profiling by array

Summary

Molluscan larval ontogeny is a highly conserved process typical of 3 principal developmental stages. A characteristic unique to each of these stages is shell design, termed prodissoconch I, prodissoconch II and dissoconch. These shells vary in morphology, mineralogy and microstructure. The discrete temporal transitions in shell biomineralization between these larval stages are utilized in this study to investigate transcriptional involvement in several distinct biomineralization events. Scanning electron microscopy and X-ray diffraction analysis of P. maxima larvae and juveniles collected throughout post-embryonic ontogenesis, document the mineralogy and microstructure of each shelled stage as well as establishing a timeline for transitions in biomineralization. P. maxima larval samples most representative of these biomineralization distinctions and transitions were analyzed for differential gene expression on the microarray platform PmaxArray 1.0. A number of transcripts are reported as differentially expressed in correlation to the mineralization events of P. maxima larval ontogeny. Some of those isolated are known shell matrix genes while others are novel, these are discussed in relation to potential shell formation roles. This interdisciplinary investigation has married the shell developments of P. maxima larval ontogeny with corresponding gene expression profiles, furthering the elucidation of shell biomineralization.

Keywords: Temporal expression profiling by array

Overall design

Microarray is used to examine the temporal differential expression of transcripts from several bivalve larval development stages including 24hrs post fertilization, 3 days, 17 days, 20 days, 23 days, 26 days, 30 days, 35 days, 40 days. Differential expression profiles for transcripts of all the temporal samples was determined based on comparison to a common reference of unfertilized eggs. Each temporal larval sample included in the study has at least 3 replicate hybridizations. Dye flips have been incorporated in the replicates. A total of 46 microarray hybridizations were performed in this investigation for differential expression analysis.

Contributor(s)

Gardner LD, Elizur A

Citation missing

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Submission date

Jan 06, 2009

Last update date

Mar 20, 2012

Contact name

Luke Gardner

E-mail(s)

lgardner@stanford.edu

Phone

8316556237

Organization name

Stanford University

Street address

120 Ocean View Blvd, Hopkins Marine Station

City

Pacific Grove

State/province

California

ZIP/Postal code

93950

Country

USA

Platforms (1)

Pinctada maxima 5K

Samples (48)

More...

GSM357769	24hrs pf vs control reference rep 1
GSM357770	24hrs pf vs control reference rep 2
GSM357771	24hrs pf vs control reference rep 3

GSM357772	24hrs pf vs control reference rep 4
GSM357773	24hrs pf vs control reference rep 5
GSM357774	24hrs pf vs control reference rep 6
GSM357775	3 days pf vs control reference rep 1
GSM357776	3 days pf vs control reference rep 2
GSM357777	3 days pf vs control reference rep 3
GSM357778	3 days pf vs control reference rep 4
GSM357779	3 days pf vs control reference rep 5
GSM357780	3 days pf vs control reference rep 6
GSM357781	17 days pf vs control reference rep 1
GSM357782	17 days pf vs control reference rep 2
GSM357783	17 days pf vs control reference rep 3
GSM357784	17 days pf vs control reference rep 4
GSM357785	17 days pf vs control reference rep 5
GSM357786	17 days pf vs control reference rep 6
GSM357787	20 days pf vs control reference rep 1
GSM357788	20 days pf vs control reference rep 2
GSM357789	20 days pf vs control reference rep 3
GSM357790	20 days pf vs control reference rep 4
GSM357791	23 days pf vs control reference rep 1
GSM357792	23 days pf vs control reference rep 2
GSM357793	23 days pf vs control reference rep 3
GSM357794	23 days pf vs control reference rep 4
GSM357795	23 days pf vs control reference rep 5
GSM357796	23 days pf vs control reference rep 6
GSM357797	23 days pf vs control reference rep 7
GSM357798	26 days pf vs control reference rep 1
GSM357799	26 days pf vs control reference rep 2
GSM357800	26 days pf vs control reference rep 3
GSM357801	26 days pf vs control reference rep 4
GSM357802	26 days pf vs control reference rep 5
GSM357803	26 days pf vs control reference rep 6
GSM357804	30 days pf vs control reference rep 1
GSM357805	30 days pf vs control reference rep 2
GSM357806	30 days pf vs control reference rep 3
GSM357807	30 days pf vs control reference rep 4
GSM357808	30 days pf vs control reference rep 5
GSM357809	30 days pf vs control reference rep 6
GSM357810	35 days pf vs control reference rep 1
GSM357811	35 days pf vs control reference rep 2
GSM357812	35 days pf vs control reference rep 3
GSM357813	35 days pf vs control reference rep 4
GSM357814	40 days pf vs control reference rep 1
GSM357815	40 days pf vs control reference rep 2
GSM357816	40 days pf vs control reference rep 3

This SubSeries is part of SuperSeries:

Global gene expression profile for pearl oyster

Relations

BioProject

Download family	Format
SOFT formatted family file(s)	SOFT
MINiML formatted family file(s)	MINiML
Series Matrix File(s)	TXT

Supplementary file	Size	Download	File type/resource
GSE14305_RAW.tar	51.1 Mb	(http)(custom)	TAR (of TXT)
Processed data included within Sample table

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