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Series GSE14269 Query DataSets for GSE14269
Status Public on Jan 03, 2009
Title Transgenerational epigenetic programming of the brain transcriptome and anxiety behavior
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Embryonic exposure to the endocrine disruptor vinclozolin during gonadal sex determination promotes an epigenetic reprogramming of the male germ-line that is associated with transgenerational adult onset disease states. Further analysis of this transgenerational phenotype on the brain demonstrated reproducible changes in the brain transcriptome three generations (F3) removed from the exposure. The transgenerational alterations in the male and female brain transcriptomes were distinct. In the males, the expression of 92 genes in the hippocampus and 276 genes in the amygdala were transgenerationally altered. In the females, the expression of 1,301 genes in the hippocampus and 172 genes in the amygdala were transgenerationally altered. Analysis of specific gene sets demonstrated that several brain signaling pathways were influenced including those involved in axon guidance and long-term potentiation. An investigation of behavior demonstrated that the vinclozolin F3 generation males had a decrease in anxiety-like behavior, while the females had an increase in anxiety-like behavior. These observations demonstrate that an embryonic exposure to an environmental compound appears to promote a reprogramming of brain development that correlates with transgenerational sex-specific alterations in the brain transcriptomes and behavior. Observations are discussed in regards to environmental and transgenerational influences on the etiology of brain disease.

Keywords: expression analysis, transgenerational changes due to vinclozolin
Overall design Male and female amygdala and hippocampus and male whole brain RNA samples from F3 generation control groups are compared to F3 generation vinclozolin-treated groups.
Web link
Contributor(s) Skinner M, Anway M, Savenkova M, Gore AC, Crews D
Citation(s) 19015723
Submission date Jan 02, 2009
Last update date Jul 31, 2017
Contact name Michael K Skinner
Organization name WSU
Department SBS
Street address Abelson 507
City Pullman
State/province WA
ZIP/Postal code 99163
Country USA
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (20)
GSM357074 F3-control adult male rat whole brain, replica 1
GSM357075 F3-control adult male rat whole brain, replica 2
GSM357076 F3-vinclozolin treated adult male rat whole brain, replica 1
BioProject PRJNA111233

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE14269_RAW.tar 50.9 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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