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| Status |
Public on May 08, 2020 |
| Title |
Site-dependent lineage preference of adipose stem cells |
| Organism |
Oryctolagus cuniculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Adult stem cells have unique properties in both proliferation and differentiation preference. However, the molecular mechanisms remain unclear. In this study, we hypothesized that the matrix microenvironment plays a critical role in the fate determination of local stem cells. Four rabbits were used to provide donor-matched adipose stem cells from either subcutaneous adipose tissue (ScAT) or infrapatellar fat pad (IPFP). Proliferation and multi-lineage differentiation were evaluated in adipose stem cells from donor-matched ScAT and IPFP. RNA sequencing (RNA-seq) and proteomics were conducted to uncover potential molecular mechanisms underlying the interaction between adipose stem cells and the local matrix microenvironment. We found that stem cells from ScAT exhibited significantly higher proliferation and adipogenic capacity compared to those from donor-matched IPFP while stem cells from IPFP displayed significantly higher chondrogenic potential compared to those from donor-matched ScAT. Our findings are strongly endorsed by supportive data from transcriptome and proteomics analyses, indicating that a unique extracellular matrix microenvironment is critical in the determination of local stem cell fate.
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| Overall design |
Donor-matched inguinal subcutaneous adipose tissue (ScAT) and infrapatellar fat pad (IPFP) were obtained from four 4-month-old New Zealand white rabbits. The harvested tissues were finely minced and digested using 0.1% collagenase, followed by culture in growth medium. The total RNA samples collected from donor-matched passage 1 IPFP-derived stem cells (IPFSCs) (n=4) and subcutaneous adipose stem cells (ScASCs) (n=4) underwent an initial quality control (QC) check. RNA sequencing was conducted for each.
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| Contributor(s) |
Tingliang W, Ryan H, Monika D, Lian Z, Aniello I, Gangqing H, Kirk H, Ming P |
| Citation(s) |
32351957 |
| Submission date |
Dec 26, 2019 |
| Last update date |
May 08, 2020 |
| Contact name |
Gangqing Hu |
| E-mail(s) |
michael.hu@hsc.wvu.edu
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| Organization name |
West Virginia University
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| Department |
MicroBiology, Immunology, and Cell Biology
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| Lab |
2072A, HSC North, Floor 2
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| Street address |
64 Medical Center Drive
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| City |
Morgantown |
| State/province |
West Virginia |
| ZIP/Postal code |
26506-9177 |
| Country |
USA |
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| Platforms (1) |
| GPL21255 |
Illumina HiSeq 2500 (Oryctolagus cuniculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA597770 |
| SRA |
SRP238843 |
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