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| Status |
Public on Jul 23, 2020 |
| Title |
RNA-seq of human and mouse brain microvessels isolated by laser capture microdissection and matched whole brain samples |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Brain microvessels form the blood-brain barrier, and are dysfunctional in several neurological disorders. Brain microvessels are formed by brain microvascular endothelial cells (BMECs) and pericytes, and the molecular constituents of these cell types remain incompletely characterized, especially in humans. To improve molecular knowledge of these cell types and identify species differences in gene expression, we performed RNA-sequencing on brain microvessels isolated from human and mouse tissue samples using laser capture microdissection. We also performed RNA-sequencing of matched whole brain samples to identify genes with microvessel-enriched expression.
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| Overall design |
A total of 12 samples were analyzed: 6 mouse samples and 6 human samples. For each species, there are 3 biological replicates of brain microvessels isolated by laser capture microdissection, and 3 matched whole brain controls.
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| Contributor(s) |
Song HW, Foreman KL, Gastfriend BD, Kuo JS, Palecek SP, Shusta EV |
| Citation(s) |
32704093 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| RC4 AA020476 |
Human Blood-Brain Barrier Model for CNS Drug Translation |
UNIVERSITY OF WISCONSIN MADISON |
ERIC V SHUSTA |
| R01 NS103844 |
Investigating Pericyte Roles in Blood-Brain Barrier Formation |
UNIVERSITY OF WISCONSIN MADISON |
ERIC V SHUSTA |
| R01 NS107461 |
Mechanisms of Shear Induction of Blood-Brain Barrier Phenotypes in Human iPSC-derived Brain Endothelial Progenitors |
UNIVERSITY OF WISCONSIN MADISON |
SEAN P PALECEK |
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| Submission date |
Dec 17, 2019 |
| Last update date |
Oct 13, 2020 |
| Contact name |
Benjamin D Gastfriend |
| Organization name |
University of California, San Diego
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| Street address |
9500 Gilman Dr
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
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| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA596224 |
| SRA |
SRP237944 |
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