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| Status |
Public on Jan 03, 2020 |
| Title |
DNase-seq profiling of Saos-2 and U2OS cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We performed DNase-seq on two osteosarcoma cell lines, Saos-2 and U2OS. This was done to identify regulatory regions within the genomes of these cells. We performed hotspot calling on these data using Hotspot2 (https://github.com/Altius/hotspot2) to identify 51,556 DNaseI hypersensitive sites within Saos-2 cells and 63,388 within U2OS cells.
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| |
|
| Overall design |
A single replicate of each of Saos-2 and U2OS cell lines was profiled using DNase-seq
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| |
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| Contributor(s) |
Hogan MS, Vulpescu NA, Maurano MT |
| Citation(s) |
35147782 |
| |
| Submission date |
Dec 17, 2019 |
| Last update date |
Feb 28, 2022 |
| Contact name |
Matthew T Maurano |
| E-mail(s) |
maurano@nyu.edu
|
| Organization name |
NYU School of Medicine
|
| Department |
Institute for Systems Genetics
|
| Lab |
Maurano
|
| Street address |
435 East 30th Street
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA596091 |
| SRA |
SRP237908 |
