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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 12, 2009 |
| Title |
WT vs Hypomethylated Forebrain Gene Expression |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
DNA methylation is a major epigenetic factor regulating genome reprogramming, cell differentiation, developmental gene expression. To understand the role DNA methylation in CNS neurons, we generated conditional Dnmt1 mutant mice that possess ~90% hypomethylated cortical and hippocampal cells in the dorsal forebrain from E13.5 on. The mutant mice were viable with a normal lifespan, but displayed severe neuronal cell death between E14.5 to 3-weeks postnatally. Accompanied with the striking cortical and hippocampal degeneration, adult mutant mice exhibited neurobehavioral defects in learning and memory in adulthood. Unexpectedly, a fraction of Dnmt1-/- cortical neurons survived through postnatal development, so that the residual cortex in mutant mice contained 20-30% of hypomethylated neurons throughout the life. Hypomethylated excitatory neurons exhibited multiple defects in postnatal maturation including abnormal dendritic arborization and impaired neuronal excitability. The mutant phenotypes are coupled with deregulation of those genes involved in neuronal layer-specification, cell death, and the function of ion channels. Our results suggest that DNA methylation, through its role in modulating neuronal gene expression, plays multiple roles in regulating cell survival, neuronal migration and maturation in the CNS.
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| Overall design |
We compared gene expression patterns in Wildtype and DNA methylation deficient (Emx1-cre; Dnmt1 mutant) mouse dorsal cortex. We performed 3 replicates using different each individual mouse strain. The Sample GSM350992 table is the average log ratio for the 3 replicatesArrays were performed in triplicate.
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| Contributor(s) |
Hutnick LK, Namihira M, Fan G |
| Citation(s) |
19433415 |
| Submission date |
Dec 24, 2008 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Masakazu Namihira |
| E-mail(s) |
namihira@bs.naist.jp
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| Organization name |
UCLA
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| Department |
Human Genetics
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| Lab |
Guoping Fan Lab
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| Street address |
695 Charles E. Young Dr.
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| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (1) |
| GSM350992 |
WT vs Hypomethylated Forebrain Gene Expression |
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| Relations |
| BioProject |
PRJNA111343 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE14216_RAW.tar |
46.2 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data provided as supplementary file |
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