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Status |
Public on Mar 31, 2020 |
Title |
5' droplet-based single cell RNA sequencing of human colorectal cancer and normal organoids |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Using 5' droplet-based single cell sequencing, we profiled single cells dervied from human colorectal cancer organoids carrying either APC mutation or RSPO fusion, and paired normal colon organoids for the later.
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Overall design |
Colorectal cancer and normal organoids were cultured in standard colon organoid medium or specified culture conditions to achieve differentiated state. Organoids were then FACS-sorted to obtain single cells and were subjected to sequencing. The raw data files have not been included due to privacy concerns.
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Contributor(s) |
Leung S |
Citation(s) |
32217638 |
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Submission date |
Dec 16, 2019 |
Last update date |
Jun 30, 2020 |
Contact name |
Suet Yi Leung |
E-mail(s) |
suetyi@hku.hk
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Organization name |
The University of Hong Kong
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Department |
Department of Pathology
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Street address |
Queen Mary Hospital, Pokfulam Road
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City |
Hong Kong |
ZIP/Postal code |
Nil |
Country |
Hong Kong |
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Platforms (1) |
GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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Samples (12)
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Relations |
BioProject |
PRJNA595931 |