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Series GSE141998 Query DataSets for GSE141998
Status Public on Jan 21, 2020
Title HDAC1 binding regions in mouse embryonic limbs
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Purpose: The response to Hedgehog signaling in the limb is driven by GLI bound enhancers and the majority of Hh targets in the developing limb bud are regulated solely by the activity of GLI-repressor. Currently we do not have a comprehensive understanding of how GLI bound enhancers respond Hedgehog signaling. The goal of this study is to identify how GLI bound enhancers are regulated by Hedgehog signaling and specifically by GLI-repressor.
Methods: ChIP-seq was performed in Embryonic day 11.5 mouse forelimb and hidlimbs lfrom wildtype Swiss Webster mice.
Results: We identified 15,347 HDAC1 binding sites in mouse E11.5 limb buds
 
Overall design ChIP-sequencing E11.5 forelimb and hindlimbs from wildtype Swiss Webster mice. Four biological replicates and three input controls were submitted.
 
Contributor(s) Lex RK, Ji Z, Ji H, Vokes SA
Citation(s) 31989924, 35145123
Submission date Dec 13, 2019
Last update date Mar 09, 2022
Contact name Steven Vokes
E-mail(s) svokes@austin.utexas.edu
Organization name The University of Texas at Austin
Department Department of Molecular Biosciences, Institute for Cellular & Molecular Biology
Street address 2500 Speedway, Stop A4800
City Austin
State/province Texas
ZIP/Postal code 78712
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (7)
GSM4217505 HDAC_ChIP_1
GSM4217506 HDAC_ChIP_2
GSM4217507 HDAC_input
This SubSeries is part of SuperSeries:
GSE108880 GLI transcriptional repression regulates enhancer activity and chromatin accessibility for Hedgehog target genes
Relations
BioProject PRJNA595550
SRA SRP237520

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE141998_HDAC1peak.csv.gz 128.8 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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