|
| Status |
Public on Jan 21, 2020 |
| Title |
HDAC1 binding regions in mouse embryonic limbs |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Purpose: The response to Hedgehog signaling in the limb is driven by GLI bound enhancers and the majority of Hh targets in the developing limb bud are regulated solely by the activity of GLI-repressor. Currently we do not have a comprehensive understanding of how GLI bound enhancers respond Hedgehog signaling. The goal of this study is to identify how GLI bound enhancers are regulated by Hedgehog signaling and specifically by GLI-repressor.
Methods: ChIP-seq was performed in Embryonic day 11.5 mouse forelimb and hidlimbs lfrom wildtype Swiss Webster mice.
Results: We identified 15,347 HDAC1 binding sites in mouse E11.5 limb buds
|
| |
|
| Overall design |
ChIP-sequencing E11.5 forelimb and hindlimbs from wildtype Swiss Webster mice. Four biological replicates and three input controls were submitted.
|
| |
|
| Contributor(s) |
Lex RK, Ji Z, Ji H, Vokes SA |
| Citation(s) |
31989924, 35145123 |
| |
| Submission date |
Dec 13, 2019 |
| Last update date |
Mar 09, 2022 |
| Contact name |
Steven Vokes |
| E-mail(s) |
svokes@austin.utexas.edu
|
| Organization name |
The University of Texas at Austin
|
| Department |
Department of Molecular Biosciences, Institute for Cellular & Molecular Biology
|
| Street address |
2500 Speedway, Stop A4800
|
| City |
Austin |
| State/province |
Texas |
| ZIP/Postal code |
78712 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (7)
|
|
| This SubSeries is part of SuperSeries: |
| GSE108880 |
GLI transcriptional repression regulates enhancer activity and chromatin accessibility for Hedgehog target genes |
|
| Relations |
| BioProject |
PRJNA595550 |
| SRA |
SRP237520 |
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