|
| Status |
Public on Dec 12, 2019 |
| Title |
Repurposing the Streptococcus mutans CRISPR-Cas9 system to understand essential gene function |
| Organism |
Streptococcus mutans |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA-seq was utilized to compare the transcriptomes of S. mutans strains that had or hadn't experienced CRISPR interference of a green fluorescent protein gene
|
| |
|
| Overall design |
Streptococcus mutans mRNA profiles were generated for a CRISPRi strain without induction, CRISPRi with induction, and a control strain lacking a sgRNA, by deep sequencing, in triplicate
|
| |
|
| Contributor(s) |
Shields RC, Chakraborty B |
| Citation(s) |
32150575 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 DE013239 |
Genetics and Physiology of Oral Biofilms |
UNIVERSITY OF FLORIDA |
Robert A Burne |
|
| |
| Submission date |
Dec 11, 2019 |
| Last update date |
Mar 12, 2020 |
| Contact name |
Robert Colquhoun Shields |
| E-mail(s) |
rshields@astate.edu
|
| Organization name |
Arkansas State University
|
| Department |
Biological Sciences
|
| Lab |
Shields
|
| Street address |
Lab Science East
|
| City |
Jonesboro |
| State/province |
Arkansas |
| ZIP/Postal code |
72467 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21130 |
Illumina NextSeq 500 (Streptococcus mutans) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA594976 |
| SRA |
SRP236893 |
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