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Series GSE141809 Query DataSets for GSE141809
Status Public on Dec 12, 2019
Title Enteric Nervous System Derived IL-18 Orchestrates Mucosal Barrier Immunity [DropSeq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Using DropSeq single cell RNA sequencing, we report that neuronal derived-IL-18 is required for goblet cell expression of intestinal antimicrobial protein expression
Mucosal barrier immunity is essential for the maintenance of the commensal microflora and combating invasive bacterial infection. Although immune and epithelial cells are thought to be the canonical orchestrators of this complex equilibrium, here we show that the enteric nervous system (ENS) plays an essential and non-redundant role in governing the anti-microbial protein (AMP) response. Using confocal microscopy and single-molecule fluorescence in situ mRNA-hybridization (smFISH) studies, we observed that intestinal neurons produce the pleiotropic cytokine IL-18. Strikingly, deletion of IL-18 from the enteric neurons alone, but not immune or epithelial cells, rendered mice susceptible to invasive Salmonella typhimurium (S.t.) infection. Mechanistically, unbiased RNA sequencing and single cell sequencing revealed that enteric neuronal IL-18 is specifically required for homeostatic goblet cell AMP production. Together, we show that neuron derived IL-18 signaling controls tissue wide intestinal immunity and has profound consequences on the mucosal barrier and invasive bacterial killing.
 
Overall design Colons from littermate steady state Il18f/f and Il18f/fHand2Cre+ mice were digested and single cell suspension generated and DropSeq conducted. Sample 5 is collected from Il18f/f mice (n=2 combined) and Sample 6 is collected from Il18f/fHand2Cre+ mice (n=2 combined).
 
Contributor(s) Flavell RA
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Submission date Dec 11, 2019
Last update date Jan 27, 2020
Contact name Richard Flavell
E-mail(s) richard.flavell@yale.edu
Organization name Yale University
Department Department of Immunobiology
Street address 300 Cedar Street, TAC S-569
City New Haven
State/province CT
ZIP/Postal code 06511
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (2)
GSM4213612 Sample_5
GSM4213613 Sample_6
This SubSeries is part of SuperSeries:
GSE141810 Enteric Nervous System Derived IL-18 Orchestrates Mucosal Barrier Immunity
Relations
BioProject PRJNA594860
SRA SRP236594

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE141809_RAW.tar 4.5 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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