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| Status |
Public on Sep 12, 2020 |
| Title |
isoCirc catalogs full-length circular RNA isoforms in human transcriptomes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report isoCirc, a long-read sequencing strategy coupled with an integrated computational pipeline to characterize full-length circular RNA (circRNA isoforms) using rolling circle amplification (RCA) followed by long-read sequencing. Applying isoCirc to 12 human tissues, we determined full-length structures and examined tissue specificities of circRNA isoforms in human transcriptomes.
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| Overall design |
Short-read (Illumina) and long-read (Oxford Nanopore, ONT) sequencing were used to examine circRNA isoforms in total RNA extracted from human embryonic kidney (HEK293) cells (6 replicates). Long-read sequencing was used to examine circRNA isoforms in total RNA extracted from 12 human tissues.
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| |
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| Contributor(s) |
Xin R, Gao Y, Wang R, Kadash-Edmondson KE, Xing Y |
| Citation(s) |
33436621 |
| |
| Submission date |
Dec 09, 2019 |
| Last update date |
Jan 27, 2021 |
| Contact name |
Yi Xing |
| E-mail(s) |
xingyi@email.chop.edu
|
| Organization name |
Children's Hospital of Philadelphia
|
| Department |
Department of Pathology and Laboratory Medicine
|
| Street address |
3615 Civic Center Blvd
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (2) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA594380 |
| SRA |
SRP235284 |
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