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| Status |
Public on Dec 28, 2022 |
| Title |
Gene expression changes in undifferentiated and differentiated human primary epithelial cells of antrum, corpus, and fundus |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression changes specific for healthy undifferentiated and differentiated human gastric primary epithelial cells of different regions of the stomach were here determined in order to describe the transcriptomic profile of these cells in correlation to their DNA methylation pattern. In addition, gene expression changes upon 5-aza-2'-deoxycytidine treatment of antral gastric primary cells compared to mock-treated cells were deterimend.
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| Overall design |
Primary gastric epithelial cells were isolated from gastric sleeve resection material and cultivated as musosoids for 1-2 passages. RNA was isolated from the filter following the Trizol extraction method and gene expression was determined by Agilent 8x60k human custom single-color arrays. Three biological replicates of six different conditions (undifferentiated and differentiated antrum, corpus, and fundus samples) were analyzed as healthy gastric primary cells and two biological replicates of 5-aza-2'-deoxycytidine-treated and mock-treated undifferentiated antral samples were analyzed.
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| Contributor(s) |
Fritsche K, Berger H, Meyer TF |
| Citation(s) |
36585699 |
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| Submission date |
Dec 09, 2019 |
| Last update date |
Jan 11, 2023 |
| Contact name |
Thomas F Meyer |
| E-mail(s) |
tfm@mpiib-berlin.mpg.de
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| Phone |
03028 460 404
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| Organization name |
Max Planck Institute for Infection Biology
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| Department |
Molecular Biology
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| Street address |
Chariteplatz 1
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| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
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| Platforms (1) |
| GPL23572 |
Agilent-048908 8x60K whole genome incl V1-V2 linc BioVacSafe final 048908 [Probe Name version] |
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| Samples (22)
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| This SubSeries is part of SuperSeries: |
| GSE141660 |
Changes in undifferentiated and differentiated human primary epithelial cells of antrum, corpus, and fundus |
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| Relations |
| BioProject |
PRJNA594315 |
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