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| Status |
Public on Oct 29, 2021 |
| Title |
RNA-seq analysis of mRNA profiles in the livers of Mettl3 flox/flox and hepatocyte-specific Mettl3 knockout (HKO) mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: The goal of this study is to compare transcriptome profilings of liver from Mettl3 flox/flox and hepatocyte-specific Mettl3 knockout (HKO) mice.
Methods: Total RNA was extracted using Tripure Isolation Reagent (Roche, Mannheim, Germany) from livers of Mettl3 flox/flox and HKO mice at 8 weeks old. mRNA profiles were generated by deep sequencing using an Illumina HiSeq X Ten platform. Paired-end clean reads were aligned to the mouse reference genome(Ensemble_GRCm38.90) with Hisat2 (version 2.0.4), and the aligned reads were used to quantify mRNA expression by using HTSeq (version 0.9.1).
Conclusion: The hepatic mRNA profiles in Mettl3 flox/flox and HKO mice were characterized.
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| Overall design |
RNA samples were pooled from livers of METTL3 HKO and Mettl3 flox/flox mice (n=4 for each group). mRNA profiles of HKO and Mettl3 flox/flox livers were generated by deep sequencing, in a single test, using Illumina HiSeq X Ten platform.
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| Contributor(s) |
Chen Z, Li X |
| Citation(s) |
34893641, 35927268 |
| |
| Submission date |
Nov 30, 2019 |
| Last update date |
Aug 16, 2022 |
| Contact name |
Zheng Chen |
| E-mail(s) |
chenzheng@hit.edu.cn
|
| Phone |
+8645186402029
|
| Organization name |
Harbin Institute of Technology
|
| Street address |
92 Xidazhijie Harbin Institute of Technology
|
| City |
Harbin |
| State/province |
Heilongjiang |
| ZIP/Postal code |
150001 |
| Country |
China |
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| Platforms (1) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE141325 |
RNA-seq and ATAC-seq of liver samples in Mettl3 flox/flox and hepatocyte-specific Mettl3 knockout (HKO) mice |
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| Relations |
| BioProject |
PRJNA592704 |
| SRA |
SRP234074 |
