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| Status |
Public on Nov 22, 2019 |
| Title |
The short time-series transcriptome profiles of a human adipose mesenchymal stem cell by electrical stimulation of enzymatic biofuel cell |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We investigate the mRNA expression properties of hAD-MSCs by electrical stimulation using EBFC as an electrical regulator. We set up three different electrical currents, at 120±10.2, 250±11.5, and 500±16.2 nA/cm2 in hAD-MSCs. We performed transcriptome profiling to analyze the impact of EBFC-derived electrical current on gene expression using next-generation sequencing (NGS).
Among the 166 up-regulated genes, 98 genes were significantly enriched into the UniProtKB functional categories such as ribosomal protein, alternative splicing, ribonucleoprotein, and amino-acid biosynthesis. This indicate that the genes highly associated with metabolic regulation of cell growth and proliferation have been changed by electrical stimulation in the EBFCs.
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| Overall design |
mRNA profiles of wild-type human adipose-derived mesenchymal stem cells (hAD-MSCs) and hAD-MSCs under nano-scale electrical stimulation at 120±10.2, 250±11.5, and 500±16.2 nA/cm2, using Illumina HiSeq2500.
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| Contributor(s) |
Kang K, Han K, Mun S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 21, 2019 |
| Last update date |
Nov 27, 2019 |
| Contact name |
Jae Ho Lee |
| E-mail(s) |
drjaeho@gmail.com
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| Organization name |
CHA University
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| Department |
Department of biomedical Science
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| Street address |
120, Haeryong-ro
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| City |
Pocheon-si |
| State/province |
Gyeonggi-do |
| ZIP/Postal code |
11160 |
| Country |
South Korea |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA590948 |
| SRA |
SRP231149 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE140781_CDrefFlat_RPKM_table_Submit.txt.gz |
381.3 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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